Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-10-19
pubmed:abstractText
Engagement of the Fcepsilon receptor I (FcepsilonRI) on mast cells and basophils initiates signaling pathways leading to degranulation. Early activation events include tyrosine phosphorylation of two transmembrane adaptor proteins, linker for activation of T cells (LAT) and non-T cell activation linker (NTAL; also called LAB; a product of Wbscr5 gene). Previous studies showed that the secretory response was partially inhibited in bone marrow-derived mast cells (BMMCs) from LAT-deficient mice. To clarify the role of NTAL in mast cell degranulation, we compared FcepsilonRI-mediated signaling events in BMMCs from NTAL-deficient and wild-type mice. Although NTAL is structurally similar to LAT, antigen-mediated degranulation responses were unexpectedly increased in NTAL-deficient mast cells. The earliest event affected was enhanced tyrosine phosphorylation of LAT in antigen-activated cells. This was accompanied by enhanced tyrosine phosphorylation and enzymatic activity of phospholipase C gamma1 and phospholipase C gamma2, resulting in elevated levels of inositol 1,4,5-trisphosphate and free intracellular Ca2+. NTAL-deficient BMMCs also exhibited an enhanced activity of phosphatidylinositol 3-OH kinase and Src homology 2 domain-containing protein tyrosine phosphatase-2. Although both LAT and NTAL are considered to be localized in membrane rafts, immunogold electron microscopy on isolated membrane sheets demonstrated their independent clustering. The combined data show that NTAL is functionally and topographically different from LAT.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-10204488, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-10358778, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-10360968, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-10404150, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-10831616, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-10843385, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11169432, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11449275, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11489921, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11493662, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11593409, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11713268, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11768780, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-11971018, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-12024020, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-12089510, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-12486104, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-12514734, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-12970761, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-14500655, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-15010370, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-15030568, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-15034144, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-15153499, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-15162432, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-1696892, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-2971137, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-6790293, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-7373045, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-8559668, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-8647120, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-9034145, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-9464827, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-9846483, http://linkedlifedata.com/resource/pubmed/commentcorrection/15477348-9880667
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing, http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular..., http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/LAB protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Lat protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases, http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Wbscr5 protein, mouse
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1001-13
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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