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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-1-10
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pubmed:abstractText |
The goal of this study was to examine whether IL-6 could directly protect lung resident cells, especially alveolar epithelial cells, from reactive oxygen species (ROS)-induced cell death. ROS induced IL-6 gene expression in organotypic lung slices of wild-type (WT) mice. ROS also induced IL-6 gene expression in mouse primary lung fibroblasts, dose dependently. The organotypic lung slices of WT were more resistant to ROS-induced DNA fragmentation than those of IL-6-deficient (IL-6-/-) mice. WT resistance against ROS was abrogated by treatment with anti-IL-6 antibody. TdT-mediated dUTP nick end labeling stain and electron microscopy revealed that DNA fragmented cells in the IL-6-/- slice included alveolar epithelial cells and endothelial cells. In vitro studies demonstrated that IL-6 reduced ROS-induced A549 alveolar epithelial cell death. Together, these data suggest that IL-6 played an antioxidant role in the lung by protecting lung resident cells, especially alveolar epithelial cells, from ROS-induced cell death.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1040-0605
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pubmed:author |
pubmed-author:HoshinoShigenoriS,
pubmed-author:InoueKojiK,
pubmed-author:KawaseIchiroI,
pubmed-author:KidaHiroshiH,
pubmed-author:KumagaiToruT,
pubmed-author:OsakiTadashiT,
pubmed-author:SaekiYukihikoY,
pubmed-author:TachibanaIsaoI,
pubmed-author:YanagitaMasahikoM,
pubmed-author:YanoYukihiroY,
pubmed-author:YoshidaMitsuhiroM
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pubmed:issnType |
Print
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pubmed:volume |
288
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L342-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15475383-Animals,
pubmed-meshheading:15475383-Antibodies, Monoclonal,
pubmed-meshheading:15475383-Cell Survival,
pubmed-meshheading:15475383-Cells, Cultured,
pubmed-meshheading:15475383-Cytoprotection,
pubmed-meshheading:15475383-DNA Fragmentation,
pubmed-meshheading:15475383-Epithelial Cells,
pubmed-meshheading:15475383-Fibroblasts,
pubmed-meshheading:15475383-Hydrogen Peroxide,
pubmed-meshheading:15475383-Interleukin-6,
pubmed-meshheading:15475383-Male,
pubmed-meshheading:15475383-Mice,
pubmed-meshheading:15475383-Mice, Inbred C57BL,
pubmed-meshheading:15475383-Mice, Knockout,
pubmed-meshheading:15475383-Organ Culture Techniques,
pubmed-meshheading:15475383-Oxidants,
pubmed-meshheading:15475383-Oxidative Stress,
pubmed-meshheading:15475383-Pulmonary Alveoli,
pubmed-meshheading:15475383-RNA, Messenger,
pubmed-meshheading:15475383-Reactive Oxygen Species
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pubmed:year |
2005
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pubmed:articleTitle |
Protective effect of IL-6 on alveolar epithelial cell death induced by hydrogen peroxide.
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pubmed:affiliation |
National Hospital Organization Japan, Osaka Minami Medical Center, Kawachinagano, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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