| pubmed-article:15475249 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C0056208 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C1880945 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:15475249 | lifeskim:mentions | umls-concept:C1880156 | lld:lifeskim |
| pubmed-article:15475249 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15475249 | pubmed:dateCreated | 2004-10-11 | lld:pubmed |
| pubmed-article:15475249 | pubmed:abstractText | We developed an integrated system suitable for comprehensive gene expression studies including construction and analysis of cDNA microarrays starting from a small amount of mRNA. We amplified total mRNA first as cDNA mixtures by polymerase chain reaction and then as strand-specific cRNA mixtures by in vitro transcription. These amplified cDNA and cRNA enabled determination of mRNA levels by hybridization analyses such as Southern, Northern, reverse-Northern macroarray, and cDNA microarray analyses, as well as construction of the cDNA library with a unidirectional cDNA insert. By using strand-specific cRNA derived from rat primary-cultured hepatocytes, we detected putative antisense transcripts for the metallothionein gene. cDNA microarray analysis for genes regulated by glucocorticoids and glucagon in the hepatocytes revealed that a number of genes involved in signal transduction and transcriptional regulation were up- or down-regulated. The present system is widely applicable to gene expression analysis with limited amounts of RNA samples. | lld:pubmed |
| pubmed-article:15475249 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15475249 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15475249 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15475249 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:15475249 | pubmed:issn | 0888-7543 | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:SakaoEikoE | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:TakiguchiMasa... | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:MiyauchiOsamu... | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:KohnoYoichiY | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:KatoMasakiM | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:SekiNaohikoN | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:KanazawaMasak... | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:YamamotoShige... | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:OhtsukaSatoko... | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:Shimizu-YabeA... | lld:pubmed |
| pubmed-article:15475249 | pubmed:author | pubmed-author:IwaseKatsuroK | lld:pubmed |
| pubmed-article:15475249 | pubmed:copyrightInfo | Copyright 2004 Elsevier Inc. | lld:pubmed |
| pubmed-article:15475249 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15475249 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:15475249 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15475249 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15475249 | pubmed:pagination | 715-29 | lld:pubmed |
| pubmed-article:15475249 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:15475249 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15475249 | pubmed:articleTitle | An mRNA amplification procedure with directional cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis. | lld:pubmed |
| pubmed-article:15475249 | pubmed:affiliation | Department of Biochemistry and Genetics, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan. | lld:pubmed |
| pubmed-article:15475249 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15475249 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15475249 | lld:pubmed |
