|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2004-10-11
|
|
pubmed:abstractText |
We developed an integrated system suitable for comprehensive gene expression studies including construction and analysis of cDNA microarrays starting from a small amount of mRNA. We amplified total mRNA first as cDNA mixtures by polymerase chain reaction and then as strand-specific cRNA mixtures by in vitro transcription. These amplified cDNA and cRNA enabled determination of mRNA levels by hybridization analyses such as Southern, Northern, reverse-Northern macroarray, and cDNA microarray analyses, as well as construction of the cDNA library with a unidirectional cDNA insert. By using strand-specific cRNA derived from rat primary-cultured hepatocytes, we detected putative antisense transcripts for the metallothionein gene. cDNA microarray analysis for genes regulated by glucocorticoids and glucagon in the hepatocytes revealed that a number of genes involved in signal transduction and transcriptional regulation were up- or down-regulated. The present system is widely applicable to gene expression analysis with limited amounts of RNA samples.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0888-7543
|
|
pubmed:author |
pubmed-author:IwaseKatsuroK,
pubmed-author:KanazawaMasakiM,
pubmed-author:KatoMasakiM,
pubmed-author:KohnoYoichiY,
pubmed-author:MiyauchiOsamuO,
pubmed-author:OhtsukaSatokoS,
pubmed-author:SakaoEikoE,
pubmed-author:SekiNaohikoN,
pubmed-author:Shimizu-YabeAtsukoA,
pubmed-author:TakiguchiMasakiM,
pubmed-author:YamamotoShigenoriS
|
|
pubmed:copyrightInfo |
Copyright 2004 Elsevier Inc.
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
84
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
715-29
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15475249-Animals,
pubmed-meshheading:15475249-Base Sequence,
pubmed-meshheading:15475249-Cells, Cultured,
pubmed-meshheading:15475249-Cloning, Molecular,
pubmed-meshheading:15475249-DNA, Complementary,
pubmed-meshheading:15475249-Gene Amplification,
pubmed-meshheading:15475249-Gene Expression,
pubmed-meshheading:15475249-Gene Library,
pubmed-meshheading:15475249-Glucagon,
pubmed-meshheading:15475249-Glucocorticoids,
pubmed-meshheading:15475249-Hepatocytes,
pubmed-meshheading:15475249-Metallothionein,
pubmed-meshheading:15475249-Mice,
pubmed-meshheading:15475249-Molecular Sequence Data,
pubmed-meshheading:15475249-Neurons,
pubmed-meshheading:15475249-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15475249-Polymerase Chain Reaction,
pubmed-meshheading:15475249-RNA, Antisense,
pubmed-meshheading:15475249-RNA, Complementary,
pubmed-meshheading:15475249-RNA, Messenger,
pubmed-meshheading:15475249-Rats,
pubmed-meshheading:15475249-Transcription, Genetic
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
An mRNA amplification procedure with directional cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis.
|
|
pubmed:affiliation |
Department of Biochemistry and Genetics, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
