| pubmed-article:15474704 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15474704 | lifeskim:mentions | umls-concept:C0038027 | lld:lifeskim |
| pubmed-article:15474704 | lifeskim:mentions | umls-concept:C0004595 | lld:lifeskim |
| pubmed-article:15474704 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:15474704 | lifeskim:mentions | umls-concept:C0442027 | lld:lifeskim |
| pubmed-article:15474704 | pubmed:issue | 31-32 | lld:pubmed |
| pubmed-article:15474704 | pubmed:dateCreated | 2004-10-11 | lld:pubmed |
| pubmed-article:15474704 | pubmed:abstractText | Recombinant Bacillus subtilis spores were employed as a vaccine delivery system in a heterologous mucosal priming-parenteral boosting vaccination strategy in the mouse model. BALB/c and C57BL/6 mice were orally immunised with recombinant spores expressing tetanus toxin fragment C (TTFC) fused to the spore outer coat protein CotB, and then subcutaneously boosted with soluble TTFC (without adjuvant). Two weeks after boosting, a significantly higher serum TTFC-specific IgG response was stimulated in mice primed with recombinant spores (antibody concentration of 2600 +/- 915 in C57BL/6 and 1200 +/- 370 ng/ml in BALB/c) compared to mice inoculated with wild type spores (650 +/- 250 and 250 +/- 130 ng/ml, respectively). IgG subclass analysis showed a prevalence of IgG1 and IgG2b, indicative of a Th2 type of immune response. Oral administration of recombinant spores stimulated also a significant local TTFC-specific IgA response. These data show that recombinant spores of B. subtilis are able to prime the immune system by the oral route, and that a combined mucosal/parenteral strategy can stimulate both local and systemic antigen-specific immune responses. | lld:pubmed |
| pubmed-article:15474704 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15474704 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15474704 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15474704 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:15474704 | pubmed:issn | 0264-410X | lld:pubmed |
| pubmed-article:15474704 | pubmed:author | pubmed-author:OggioniMarco... | lld:pubmed |
| pubmed-article:15474704 | pubmed:author | pubmed-author:PozziGianniG | lld:pubmed |
| pubmed-article:15474704 | pubmed:author | pubmed-author:CiabattiniAnn... | lld:pubmed |
| pubmed-article:15474704 | pubmed:author | pubmed-author:IsticatoRache... | lld:pubmed |
| pubmed-article:15474704 | pubmed:author | pubmed-author:ParigiRiccard... | lld:pubmed |
| pubmed-article:15474704 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15474704 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:15474704 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:15474704 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15474704 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15474704 | pubmed:pagination | 4139-43 | lld:pubmed |
| pubmed-article:15474704 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:meshHeading | pubmed-meshheading:15474704... | lld:pubmed |
| pubmed-article:15474704 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15474704 | pubmed:articleTitle | Oral priming of mice by recombinant spores of Bacillus subtilis. | lld:pubmed |
| pubmed-article:15474704 | pubmed:affiliation | Laboratorio di Microbiologia Molecolare e Biotecnologia (LAMMB), Dipartimento di Biologia Molecolare, Università di Siena, Policlinico Le Scotte V lotto, piano 1, 53100 Siena, Italy. | lld:pubmed |
| pubmed-article:15474704 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15474704 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15474704 | lld:pubmed |
