Linked Life Data

15472118

Source:http://linkedlifedata.com/resource/pubmed/id/15472118

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-10-29
pubmed:abstractText
Local control of cerebral blood flow is regulated in part through myogenic constriction of resistance arteries. Although this response requires Ca2+ influx via voltage-dependent Ca2+ channels secondary to smooth muscle cell depolarization, the mechanisms responsible for alteration of vascular smooth muscle (VSM) cell membrane potential are not fully understood. A previous study from our laboratory demonstrated a critical role for a member of the transient receptor potential (TRP) superfamily of ion channels, TRPC6, in this response. Several other of the approximately 22 identified TRP proteins are also present in cerebral arteries, but their functions have not been elucidated. Two of these channels, TRPM4 and TRPM5, exhibit biophysical properties that are consistent with a role for control of membrane potential of excitable cells. We hypothesized that TRPM4/TRPM5-dependent currents contribute to myogenic vasoconstriction of cerebral arteries. Cation channels with unitary conductance, ion selectivity and Ca2+-dependence similar to those of cloned TRPM4 and TRPM5 were present in freshly isolated VSM cells. We found that TRPM4 mRNA was detected in both whole cerebral arteries and in isolated VSM cells whereas TRPM5 message was absent from cerebral artery myocytes. We also found that pressure-induced smooth muscle cell depolarization was attenuated in isolated cerebral arteries treated with TRPM4 antisense oligodeoxynucleotides to downregulate channel subunit expression. In agreement with these data, myogenic vasoconstriction of intact cerebral arteries administered TRPM4 antisense was attenuated compared with controls, whereas KCl-induced constriction did not differ between groups. We concluded that activation of TRPM4-dependent currents contributed to myogenic vasoconstriction of cerebral arteries.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
29
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
922-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15472118-Animals, pubmed-meshheading:15472118-Arteries, pubmed-meshheading:15472118-Calcium, pubmed-meshheading:15472118-Cerebellum, pubmed-meshheading:15472118-Cerebral Arteries, pubmed-meshheading:15472118-Down-Regulation, pubmed-meshheading:15472118-Ion Channels, pubmed-meshheading:15472118-Ion Transport, pubmed-meshheading:15472118-Male, pubmed-meshheading:15472118-Membrane Potentials, pubmed-meshheading:15472118-Muscle, Smooth, Vascular, pubmed-meshheading:15472118-Myocytes, Smooth Muscle, pubmed-meshheading:15472118-Oligodeoxyribonucleotides, pubmed-meshheading:15472118-Oligodeoxyribonucleotides, Antisense, pubmed-meshheading:15472118-Patch-Clamp Techniques, pubmed-meshheading:15472118-RNA, Messenger, pubmed-meshheading:15472118-Rats, pubmed-meshheading:15472118-Rats, Sprague-Dawley, pubmed-meshheading:15472118-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15472118-TRPM Cation Channels, pubmed-meshheading:15472118-Tetradecanoylphorbol Acetate, pubmed-meshheading:15472118-Uridine Triphosphate, pubmed-meshheading:15472118-Vasoconstriction
pubmed:year
2004
pubmed:articleTitle
Critical role for transient receptor potential channel TRPM4 in myogenic constriction of cerebral arteries.
pubmed:affiliation
Department of Pharmacology, University of Vermont College of Medicine, 89 Beaumont Ave, Burlington, VT, USA. Scott.Earley@uvm.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.