Linked Life Data

15471558

Source:http://linkedlifedata.com/resource/pubmed/id/15471558

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-10-8
pubmed:abstractText
We have previously demonstrated significantly decreased immunoreactivity of hepatocyte growth factor activator inhibitor type 1 (HAI-1), an integral membrane protein that exhibits potent inhibitory activity against hepatocyte growth factor activator (HGFA) and matriptase, in colorectal adenocarcinomas. In this report, we describe further detailed analysis of HAI-1 expression in colorectal adenocarcinoma by using three kinds of anti-HAI-1 antibodies, each of which recognizes a distinct epitope of the HAI-1 molecule, and also by in-situ hybridization for HAI-1 mRNA. The results indicated that the decreased immunoreactivity of HAI-1 in colorectal carcinoma cells is largely a result of enhanced ectodomain shedding of HAI-1 in these cells. In contrast, immunoreactivity of mature membrane-form HAI-1 was paradoxically en-hanced in cancer cells at the invasion front, showing intense cell-stroma interactions and/or sprouting invasion. This finding indicates that these invading cells showed decreased ectodomain shedding of HAI-1 and consequently might require the existence of the membrane-form HAI-1. Of particular interest was the observation of a possible inverse correlation between paradoxical up-regulation of membrane-form HAI-1 expression and membrane-associated E-cadherin in these cells. These membrane-form HAI-1-positive sprouting cancer cells were also negative for MIB-1 immunohistochemically, indicating a low-proliferating population. All these results suggest that HAI-1 may mediate diverse functions in regard to the progression of colorectal carcinomas, and the immunoreactivity of membrane-form HAI-1 may serve as a marker of invading cancer cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1347-9032
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
728-35
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15471558-Adenocarcinoma, pubmed-meshheading:15471558-Adult, pubmed-meshheading:15471558-Aged, pubmed-meshheading:15471558-Aged, 80 and over, pubmed-meshheading:15471558-Animals, pubmed-meshheading:15471558-Cadherins, pubmed-meshheading:15471558-Cell Membrane, pubmed-meshheading:15471558-Colorectal Neoplasms, pubmed-meshheading:15471558-Disease Progression, pubmed-meshheading:15471558-Epitopes, pubmed-meshheading:15471558-Female, pubmed-meshheading:15471558-Humans, pubmed-meshheading:15471558-Immunoglobulin G, pubmed-meshheading:15471558-In Situ Hybridization, pubmed-meshheading:15471558-Male, pubmed-meshheading:15471558-Membrane Glycoproteins, pubmed-meshheading:15471558-Middle Aged, pubmed-meshheading:15471558-Neoplasm Invasiveness, pubmed-meshheading:15471558-Peptide Fragments, pubmed-meshheading:15471558-Proteinase Inhibitory Proteins, Secretory, pubmed-meshheading:15471558-RNA, Messenger, pubmed-meshheading:15471558-Rabbits
pubmed:year
2004
pubmed:articleTitle
Paradoxically enhanced immunoreactivity of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in cancer cells at the invasion front.
pubmed:affiliation
Second Department of Pathology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't