Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2004-10-7
pubmed:abstractText
The Cre/loxP recombination system can be used to circumvent many of the limitations of generalized gene ablation in mice. Here we present the development and characterization of transgenic mice in which Cre recombinase has been targeted to cells of the osteoblast lineage with 2.3 kb (Col 2.3-Cre) and 3.6 kb (Col 3.6-Cre) fragments of the rat Col1a1 promoter. Cre mRNA was detected in calvaria and long bone of adult Col 2.3-Cre and Col 3.6-Cre mice, as well as in tendon and skin of Col 3.6-Cre mice. To obtain a historical marking of the temporal and spatial pattern of Cre-mediated gene rearrangement, Col-Cre mice were bred with ROSA26 (R26R) mice in which Cre-mediated excision of a floxed cassette results in LacZ expression. In Col 2.3-Cre;R26R and Col 3.6-Cre;R26R progeny, calvarial and long bone osteoblasts showed intense beta-gal staining at embryonic day 18 and postnatal day 5. The spatial pattern of beta-gal staining was more restricted in bone and in bone marrow stromal cultures established from Col 2.3-Cre;R26R mice. Similar differences in the spatial patterns of expression were seen in transgenic bone carrying Col1a1-GFP visual reporters. Our data suggest that Col 2.3-Cre and Col 3.6-Cre transgenic mice may be useful for conditional gene targeting in vivo or for obtaining osteoblast populations for in vitro culture in which a gene of interest has been inactivated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0214-6282
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
645-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15470637-Animals, pubmed-meshheading:15470637-Blotting, Northern, pubmed-meshheading:15470637-Bone Marrow Cells, pubmed-meshheading:15470637-Cells, Cultured, pubmed-meshheading:15470637-Cloning, Molecular, pubmed-meshheading:15470637-Collagen Type I, pubmed-meshheading:15470637-Genetic Techniques, pubmed-meshheading:15470637-Genotype, pubmed-meshheading:15470637-Green Fluorescent Proteins, pubmed-meshheading:15470637-Integrases, pubmed-meshheading:15470637-Mice, pubmed-meshheading:15470637-Mice, Transgenic, pubmed-meshheading:15470637-Models, Genetic, pubmed-meshheading:15470637-Muscle, Skeletal, pubmed-meshheading:15470637-Osteoblasts, pubmed-meshheading:15470637-Plasmids, pubmed-meshheading:15470637-Polymerase Chain Reaction, pubmed-meshheading:15470637-Promoter Regions, Genetic, pubmed-meshheading:15470637-RNA, pubmed-meshheading:15470637-Rats, pubmed-meshheading:15470637-Time Factors, pubmed-meshheading:15470637-Tissue Distribution, pubmed-meshheading:15470637-Transgenes, pubmed-meshheading:15470637-beta-Galactosidase
pubmed:year
2004
pubmed:articleTitle
Expression and activity of osteoblast-targeted Cre recombinase transgenes in murine skeletal tissues.
pubmed:affiliation
Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't