Linked Life Data

15469941

Source:http://linkedlifedata.com/resource/pubmed/id/15469941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
52
pubmed:dateCreated
2004-12-21
pubmed:abstractText
Carnitine palmitoyltransferase I (CPT-I) catalyzes the rate-controlling step in the pathway of mitochondrial fatty acid oxidation. Thyroid hormone will stimulate the expression of the liver isoform of CPT-I (CPT-I alpha). This induction of CPT-I alpha gene expression requires the thyroid hormone response element in the promoter and sequences within the first intron. The peroxisomal proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) is a coactivator that promotes mitochondrial biogenesis, mitochondrial fatty acid oxidation, and hepatic gluconeogenesis. In addition, PGC-1 alpha will stimulate the expression of CPT-I alpha in primary rat hepatocytes. Here we report that thyroid hormone will increase PGC-1 alpha mRNA and protein levels in rat hepatocytes. In addition, overexpression of PGC-1 alpha will enhance the thyroid hormone induction of CPT-I alpha indicating that PGC-1 alpha is a coactivator for thyroid hormone. By using chromatin immunoprecipitation assays, we show that PGC-1 alpha is associated with both the thyroid hormone response element in the CPT-I alpha gene promoter and the first intron of the CPT-I alpha gene. Our data demonstrate that PGC-1 alpha participates in the stimulation of CPT-I alpha gene expression by thyroid hormone and suggest that PGC-1 alpha is a coactivator for thyroid hormone.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53963-71
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15469941-Animals, pubmed-meshheading:15469941-Carnitine O-Palmitoyltransferase, pubmed-meshheading:15469941-Cell Line, pubmed-meshheading:15469941-Enzyme Induction, pubmed-meshheading:15469941-Gene Expression, pubmed-meshheading:15469941-Immunosorbent Techniques, pubmed-meshheading:15469941-Introns, pubmed-meshheading:15469941-Liver, pubmed-meshheading:15469941-Luciferases, pubmed-meshheading:15469941-Polymerase Chain Reaction, pubmed-meshheading:15469941-Promoter Regions, Genetic, pubmed-meshheading:15469941-RNA, Messenger, pubmed-meshheading:15469941-Rats, pubmed-meshheading:15469941-Receptors, Thyroid Hormone, pubmed-meshheading:15469941-Recombinant Fusion Proteins, pubmed-meshheading:15469941-Response Elements, pubmed-meshheading:15469941-Retinoid X Receptors, pubmed-meshheading:15469941-Transcription Factors, pubmed-meshheading:15469941-Transfection, pubmed-meshheading:15469941-Triiodothyronine
pubmed:year
2004
pubmed:articleTitle
Peroxisomal proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) enhances the thyroid hormone induction of carnitine palmitoyltransferase I (CPT-I alpha).
pubmed:affiliation
Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.