Linked Life Data

15467755

Source:http://linkedlifedata.com/resource/pubmed/id/15467755

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
53
pubmed:dateCreated
2004-11-11
pubmed:abstractText
Vascular endothelial growth factor (VEGF) is an angiogenic cytokine, which plays a major role in tumor angiogenesis. VEGF mRNA expression is controlled by hypoxia, growth factors and hormones through both transcriptional and post-transcriptional mechanisms. VEGF mRNA has a short half-life and its abundance is regulated by the binding of stabilizing (HuR, hRNP-L) and still uncharacterised destabilizing proteins to its 3'-untranslated region. Here, we report that the ACTH-regulated zinc-finger protein TIS11b and its homologs TIS11 and TIS11d interact with the 3'-untranslated region of VEGF mRNA and decrease its stability (half-life reduced from 130 to 60 min). Within the 2201 bp 3'-untranslated region of VEGF mRNA, we identified a 75 bp domain, containing two consensus AU-rich motifs, which binds TIS11b and mediates its destabilizing activity. Ribonucleoprotein (RNP) complex immunoprecipitation experiments allowed us to demonstrate that the interaction between TIS11b and VEGF 3'-untranslated region occurs in live cells. Knocking down TIS11b expression in primary adrenocortical cells with small interfering (si)RNAs clearly indicated that TIS11b participates in the control of both basal and, to a larger extent, ACTH-induced VEGF mRNA expression levels. TIS11b is the first VEGF mRNA-destabilizing protein identified so far and therefore appears as a new potential target in antiangiogenic therapies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8673-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15467755-3' Untranslated Regions, pubmed-meshheading:15467755-Animals, pubmed-meshheading:15467755-Base Sequence, pubmed-meshheading:15467755-Binding Sites, pubmed-meshheading:15467755-Butyrate Response Factor 1, pubmed-meshheading:15467755-Cattle, pubmed-meshheading:15467755-DNA-Binding Proteins, pubmed-meshheading:15467755-Half-Life, pubmed-meshheading:15467755-Immediate-Early Proteins, pubmed-meshheading:15467755-Mice, pubmed-meshheading:15467755-Molecular Sequence Data, pubmed-meshheading:15467755-NIH 3T3 Cells, pubmed-meshheading:15467755-RNA, Messenger, pubmed-meshheading:15467755-RNA Interference, pubmed-meshheading:15467755-RNA Stability, pubmed-meshheading:15467755-Rats, pubmed-meshheading:15467755-Tristetraprolin, pubmed-meshheading:15467755-Vascular Endothelial Growth Factor A
pubmed:year
2004
pubmed:articleTitle
Destabilization of vascular endothelial growth factor mRNA by the zinc-finger protein TIS11b.
pubmed:affiliation
INSERM EMI 01-05, Department of Cellular Responses and Dynamics, Commissariat à l'Energie Atomique, 17 Rue des Martyrs, F-38054 Grenoble Cedex 9, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't