Linked Life Data

15467013

Source:http://linkedlifedata.com/resource/pubmed/id/15467013

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-16
pubmed:abstractText
The chemokine macrophage inflammatory protein (MIP)-1alpha recruits macrophages to sites of epithelial remodeling. We showed previously that mRNA and protein levels of MIP-1alpha in the olfactory epithelium (OE) increased significantly at 3 days after bilateral olfactory bulbectomy (OBX). The first aim of this study was to investigate the effect of the absence of MIP-1alpha on macrophage recruitment to the OE 3 days after OBX in Mip-1alpha(-/-) mice compared with C57BL/6 mice and to test whether chemokine function could be restored by MIP-1alpha protein injection into Mip-1alpha(-/-) mice. OBX was performed on C57BL/6 and Mip-1alpha(-/-) mice. The mice received six subcutaneous injections at 12-h intervals of either 10 mug/ml MIP-1alpha protein in carrier or carrier only. Macrophage recruitment was evaluated with antibodies to CD68 for all macrophages and F4/80 for activated macrophages. Compared with C57BL/6 mice, at 3 days post-OBX the numbers of CD68(+) and F4/80(+) macrophages were significantly lower in carrier-injected Mip-1alpha(-/-) mice and were comparable in MIP-1alpha protein-injected Mip-1alpha(-/-) mice. The second aim was to determine the identity of genes regulated at 3 days post-OBX in the OE of carrier-injected Mip-1alpha(-/-) mice compared with carrier-injected C57BL/6 mice. Total RNA from the OE was hybridized to Affymetrix microarrays. A number of chemokine-, cytokine-, and growth factor-related genes were significantly regulated in the Mip-1alpha(-/-) mice and were restored in MIP-1alpha protein-injected Mip-1alpha(-/-) mice. The results illustrated that MIP-1alpha played a key role in recruitment of macrophages to the OE and provided insight into the genomic regulation involved in OE remodeling.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1531-2267
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-86
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15467013-Animals, pubmed-meshheading:15467013-Antigens, CD, pubmed-meshheading:15467013-Antigens, Differentiation, Myelomonocytic, pubmed-meshheading:15467013-Bromodeoxyuridine, pubmed-meshheading:15467013-Cell Proliferation, pubmed-meshheading:15467013-Chemokine CCL3, pubmed-meshheading:15467013-Chemokine CCL4, pubmed-meshheading:15467013-Chemokines, pubmed-meshheading:15467013-Cytoskeleton, pubmed-meshheading:15467013-Immunohistochemistry, pubmed-meshheading:15467013-Macrophage Inflammatory Proteins, pubmed-meshheading:15467013-Macrophages, pubmed-meshheading:15467013-Male, pubmed-meshheading:15467013-Mice, pubmed-meshheading:15467013-Mice, Inbred C57BL, pubmed-meshheading:15467013-Mice, Transgenic, pubmed-meshheading:15467013-Nucleic Acid Hybridization, pubmed-meshheading:15467013-Olfactory Mucosa, pubmed-meshheading:15467013-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:15467013-Oligonucleotides, pubmed-meshheading:15467013-Phagocytosis, pubmed-meshheading:15467013-Phenotype, pubmed-meshheading:15467013-RNA, pubmed-meshheading:15467013-Receptors, Chemokine, pubmed-meshheading:15467013-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15467013-Time Factors, pubmed-meshheading:15467013-Up-Regulation
pubmed:year
2004
pubmed:articleTitle
Target ablation-induced regulation of macrophage recruitment into the olfactory epithelium of Mip-1alpha-/- mice and restoration of function by exogenous MIP-1alpha.
pubmed:affiliation
Department of Physiology, University of Kentucky, Lexington 40536-0230, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural