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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 22
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pubmed:dateCreated |
2004-10-14
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pubmed:abstractText |
Midkine is a heparin-binding growth factor that promotes the growth, survival, migration and differentiation of various target cells. So far, receptor-type protein tyrosine phosphatase zeta, low-density-lipoprotein-receptor-related protein and anaplastic lymphoma kinase have been identified as receptors for midkine. We found beta1 integrin in midkine-binding proteins from 13-day-old mouse embryos. beta1-Integrin bound to a midkine-agarose column and was eluted mostly with EDTA. Further study revealed that the alpha-subunits capable of binding to midkine were alpha4 and alpha6. Purified alpha4beta1- and alpha6beta1-integrins bound midkine. Anti-alpha4 antibody inhibited the midkine-dependent migration of osteoblastic cells, and anti-alpha6 antibody inhibited the midkine-dependent neurite outgrowth of embryonic neurons. After midkine treatment, tyrosine phosphorylation of paxillin, an integrin-associated molecule, was transiently increased in osteoblastic cells. Therefore, we concluded that alpha4beta1- and alpha6beta1-integrins are functional receptors for midkine. We observed that the low-density-lipoprotein-receptor-related-protein-6 ectodomain was immunoprecipitated with alpha6beta1-integrin and alpha4beta1-integrin. The low-density-lipoprotein-receptor-related-protein-6 ectodomain was also immunoprecipitated with receptor-type protein tyrosine phosphatase zeta. alpha4beta1- and alpha6beta1-Integrins are expected to co-operate with other midkine receptors, possibly in a multimolecular complex that contains other midkine receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha6beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Low Density Lipoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/Paxillin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptprz1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ptprz1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Pxn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pxn protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-Like Protein Tyrosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/midkine
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9533
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pubmed:author |
pubmed-author:ChenGuo-YunGY,
pubmed-author:MaedaNobuakiN,
pubmed-author:MuramatsuHisakoH,
pubmed-author:MuramatsuTakashiT,
pubmed-author:NodaMasaharuM,
pubmed-author:OdaYoshihiroY,
pubmed-author:OtteH PHP,
pubmed-author:SakaguchiNahokoN,
pubmed-author:SakumaSadatoshiS,
pubmed-author:SuzukiHiromichiH,
pubmed-author:TakadaYoshikazuY
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5405-15
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15466886-Alleles,
pubmed-meshheading:15466886-Animals,
pubmed-meshheading:15466886-Blotting, Western,
pubmed-meshheading:15466886-COS Cells,
pubmed-meshheading:15466886-Cell Line, Tumor,
pubmed-meshheading:15466886-Cell Movement,
pubmed-meshheading:15466886-Cytokines,
pubmed-meshheading:15466886-Cytoskeletal Proteins,
pubmed-meshheading:15466886-Drosophila melanogaster,
pubmed-meshheading:15466886-Embryo, Nonmammalian,
pubmed-meshheading:15466886-Gene Deletion,
pubmed-meshheading:15466886-Growth Substances,
pubmed-meshheading:15466886-Heparin,
pubmed-meshheading:15466886-Immunoprecipitation,
pubmed-meshheading:15466886-Integrin alpha4beta1,
pubmed-meshheading:15466886-Integrin alpha6beta1,
pubmed-meshheading:15466886-Low Density Lipoprotein Receptor-Related Protein-1,
pubmed-meshheading:15466886-Mice,
pubmed-meshheading:15466886-Mutation,
pubmed-meshheading:15466886-Neurons,
pubmed-meshheading:15466886-Paxillin,
pubmed-meshheading:15466886-Phosphoproteins,
pubmed-meshheading:15466886-Protein Binding,
pubmed-meshheading:15466886-Protein Structure, Tertiary,
pubmed-meshheading:15466886-Protein Tyrosine Phosphatases,
pubmed-meshheading:15466886-Rats,
pubmed-meshheading:15466886-Receptor-Like Protein Tyrosine Phosphatases, Class 5,
pubmed-meshheading:15466886-Time Factors,
pubmed-meshheading:15466886-Tyrosine
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pubmed:year |
2004
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pubmed:articleTitle |
alpha4beta1- and alpha6beta1-integrins are functional receptors for midkine, a heparin-binding growth factor.
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pubmed:affiliation |
Department of Biochemistry and Division of Disease Models, Center for Neural Disease and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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