Linked Life Data

15466510

Source:http://linkedlifedata.com/resource/pubmed/id/15466510

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-10-6
pubmed:abstractText
The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to Listeria monocytogenes. At a concentration of 6 mM eugenol was bactericidal to Lactobacillus sakei, but treatment with 0.5 M cinnamaldehyde had no significant effect. To investigate the role of interference with energy generation in the mechanism of action, the cellular and extracellular ATP levels of cells in HEPES buffer at 20 degrees C were measured. Treatment of nonenergized L. monocytogenes with 5 mM eugenol, 40 mM cinnamaldehyde, or 10 microM carbonyl cyanide m-chlorophenylhydrazone (CCCP) for 5 min prevented an increase in the cellular ATP concentration upon addition of glucose. Treatment of energized L. monocytogenes with 40 mM cinnamaldehyde or 10 microM CCCP caused a rapid decline in cellular ATP levels, but 5 mM eugenol had no effect on cellular ATP. Treatment of L. sakei with 10 mM eugenol prevented ATP generation by nonenergized cells and had no effect on the cellular ATP of energized cells. CCCP at a concentration of 100 microM had no significant effect on the cellular ATP of L. sakei. No significant changes in extracellular ATP were observed. Due to their rapidity, effects on energy generation clearly play a major role in the activity of eugenol and cinnamaldehyde at bactericidal concentrations. The possible mechanisms of inhibition of energy generation are inhibition of glucose uptake or utilization of glucose and effects on membrane permeability.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-10508096, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-10812612, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-11556910, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-11916669, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-12054452, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-12534815, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-12803558, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-1457292, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-8027009, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-9023935, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-9553796, http://linkedlifedata.com/resource/pubmed/commentcorrection/15466510-9750293
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5750-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei.
pubmed:affiliation
Department of Food Science, Faculty of Agriculture and Food Sciences, 250 Ellis Building, 13 Freedman Crescent, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't