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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
41
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pubmed:dateCreated |
2004-10-13
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pubmed:abstractText |
Although there was no impairment in IL-2 secretion and proliferation of Fyn-deficient naïve CD4 cells after stimulation with antigen and antigen-presenting cells, stimulation of these cells with anti-CD3 and anti-CD28 revealed profound defects. Crosslinking of purified wild-type naïve CD4 cells with anti-CD3 activated Lck and initiated the signaling cascade downstream of Lck, including phosphorylation of ZAP-70, LAT, and PLC-gamma1; calcium flux; and dephosphorylation and nuclear translocation of the nuclear factor of activated T cells (NFAT)p. All of these signaling events were diminished severely in Fyn-deficient naïve cells activated by CD3 crosslinking. Coaggregation of CD3 and CD4 reconstituted this Lck-dependent signaling pathway in Fyn(-/-) T cells. These results suggest that when signaling of naïve T cells is restricted to the T cell antigen receptor, Fyn plays an essential role by positive regulation of Lck activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-10409671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-10510341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-10523617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-10602026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-10648627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-11005864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-11180097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-11854473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-11970992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-12218089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-12223890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-12732664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-1316916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-1387588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-14707117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-15001705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-1516132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-1579166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-2190221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-2424993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-7826952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-7908301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-8293463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-8668212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9036949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9104812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9126747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9464819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9605142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9782124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15465914-9822663
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fyb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14859-64
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15465914-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:15465914-Animals,
pubmed-meshheading:15465914-Antigens, CD3,
pubmed-meshheading:15465914-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15465914-Calcium,
pubmed-meshheading:15465914-Cell Culture Techniques,
pubmed-meshheading:15465914-Interleukin-2,
pubmed-meshheading:15465914-Lymph Nodes,
pubmed-meshheading:15465914-Lymphocyte Activation,
pubmed-meshheading:15465914-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:15465914-Mice,
pubmed-meshheading:15465914-Mice, Knockout,
pubmed-meshheading:15465914-Mice, Transgenic,
pubmed-meshheading:15465914-Phosphorylation,
pubmed-meshheading:15465914-Receptors, Antigen, T-Cell,
pubmed-meshheading:15465914-Spleen,
pubmed-meshheading:15465914-Transcription Factor AP-1
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pubmed:year |
2004
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pubmed:articleTitle |
Activation of naïve CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling.
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pubmed:affiliation |
Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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