Source:http://linkedlifedata.com/resource/pubmed/id/15459901
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2004-11-3
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| pubmed:abstractText |
Previous studies on CTL responses in HIV-exposed uninfected individuals assumed that the patients were exposed to replicating HIV, but the possibility that the immune responses detected were primed by exposure to a defective virus or viral antigen could not be excluded. Epidemiological and laboratory analysis of a nosocomial outbreak of acute hepatitis B unequivocally allowed the identification of an HIV-1- and HBV-co-infected patient with high plasma levels of both viruses, as the source case of the epidemics. This clinical setting provided a natural model for testing the HIV-specific T cell response in patients exposed to blood from a patient with highly replicating HIV. Parenteral exposure to both viruses led to acute hepatitis B in five subjects without evidence of HIV-1 infection. Cryopreserved lymphocytes derived from three exposed patients were tested ex vivo in an ELISPOT assay for IFN-gamma release upon stimulation with peptides from structural and non-structural HIV proteins; one of the patients was also tested with four HLA/class I tetramers. Circulating HIV-specific CD8 cells were detected by tetramer staining and a high frequency of T cells were able to release IFN-gamma upon stimulation with HIV peptides, showing in vivo T cell priming by HIV. These results unequivocally demonstrate a HIV-specific cell-mediated immune response in the absence of infection after exposure to highly replicating HIV.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Surface Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0014-2980
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| pubmed:author |
pubmed-author:FerrariCarloC,
pubmed-author:MissaleGabrieleG,
pubmed-author:PapagnoLauraL,
pubmed-author:PennaAmaliaA,
pubmed-author:PieroniGiovanniG,
pubmed-author:PilliMassimoM,
pubmed-author:PinheiroSusanaS,
pubmed-author:Rowland-JonesSarahS,
pubmed-author:UggeriJacopoJ,
pubmed-author:UrbaniSimonaS,
pubmed-author:VitaliPieroP,
pubmed-author:ZerbiniAlessandroA
|
| pubmed:issnType |
Print
|
| pubmed:volume |
34
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3208-15
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15459901-Amino Acid Sequence,
pubmed-meshheading:15459901-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15459901-Cross Infection,
pubmed-meshheading:15459901-DNA, Viral,
pubmed-meshheading:15459901-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15459901-HIV Infections,
pubmed-meshheading:15459901-HIV-1,
pubmed-meshheading:15459901-Hepatitis B,
pubmed-meshheading:15459901-Hepatitis B Antibodies,
pubmed-meshheading:15459901-Hepatitis B Surface Antigens,
pubmed-meshheading:15459901-Hepatitis B virus,
pubmed-meshheading:15459901-Humans,
pubmed-meshheading:15459901-Interferon-gamma,
pubmed-meshheading:15459901-Lymphocyte Activation,
pubmed-meshheading:15459901-Oligopeptides,
pubmed-meshheading:15459901-Peptide Fragments,
pubmed-meshheading:15459901-Polymerase Chain Reaction,
pubmed-meshheading:15459901-Sequence Analysis, DNA,
pubmed-meshheading:15459901-Viremia
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| pubmed:year |
2004
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| pubmed:articleTitle |
Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection.
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| pubmed:affiliation |
Laboratorio di Immunopatologia Virale, Divisione Malattie Infettive ed Epatologia, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy. missale@tin.it
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|