15459184

Source:http://linkedlifedata.com/resource/pubmed/id/15459184

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0021118, umls-concept:C0086860, umls-concept:C0205147, umls-concept:C0443199, umls-concept:C0851285, umls-concept:C1416612, umls-concept:C1655731, umls-concept:C1706937, umls-concept:C1708936, umls-concept:C2587213
pubmed:issue	50
pubmed:dateCreated	2004-12-6
pubmed:abstractText	Antisense transcription has been shown to be one of the hierarchies that control gene expression in eukaryotes. Recently, we have documented that the mouse Kcnq1 imprinting control region (ICR) harbors bidirectional silencing property, and this feature is linked to an antisense RNA, Kcnq1ot1. In this investigation, using genomic footprinting, we have identified three NF-Y transcription factor binding sites appearing in a methylation-sensitive manner in the Kcnq1ot1 promoter. By employing a dominant negative mutant to the NF-Y transcription factor, we have shown that the NF-Y transcription factor positively regulates antisense transcription. Selective mutation of the conserved nucleotides in the NF-Y binding sites resulted in the loss of antisense transcription. The loss of antisense transcription from the Kcnq1ot1 promoter coincides with an enrichment in the levels of deacetylation and methylation at the lysine 9 residue of histone H3 and DNA methylation at the CpG residues, implying a crucial role for the NF-Y transcription factor in organizing the parent of origin-specific chromatin conformation in the Kcnq1 ICR. Parallel to the loss of antisense transcription, the loss of silencing of the flanking reporter genes was observed, suggesting that NF-Y-mediated Kcnq1ot1 transcription is critical in the bidirectional silencing process of the Kcnq1 ICR. These data highlight the NF-Y transcription factor as a crucial regulator of antisense promoter-mediated bidirectional silencing and the parent of origin-specific epigenetic marks at the Kcnq1 ICR. More importantly, for the first time, we document that NF-Y is involved in maintaining the antisense promoter activity against strong silencing conditions.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985121R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antisense Elements (Genetics), http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Binding Factor, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/KCNQ Potassium Channels, http://linkedlifedata.com/resource/pubmed/chemical/KCNQ1 Potassium Channel, http://linkedlifedata.com/resource/pubmed/chemical/KCNQ1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/KCNQ1OT1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Kcnq1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Voltage-Gated, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
pubmed:status	MEDLINE
pubmed:month	Dec
pubmed:issn	0021-9258
pubmed:author	pubmed-author:CeribelliMicheleM, pubmed-author:EricssonJohanJ, pubmed-author:KanduriChandrasekharC, pubmed-author:MantovaniRobertoR, pubmed-author:PandeyRadha RamanRR, pubmed-author:SinghPrim BPB
pubmed:issnType	Print
pubmed:day	10
pubmed:volume	279
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	52685-93
pubmed:dateRevised	2008-11-21
pubmed:meshHeading	pubmed-meshheading:15459184-Animals, pubmed-meshheading:15459184-Antisense Elements (Genetics), pubmed-meshheading:15459184-Base Sequence, pubmed-meshheading:15459184-Binding Sites, pubmed-meshheading:15459184-CCAAT-Binding Factor, pubmed-meshheading:15459184-Cell Line, pubmed-meshheading:15459184-DNA, pubmed-meshheading:15459184-Epigenesis, Genetic, pubmed-meshheading:15459184-Gene Expression Regulation, pubmed-meshheading:15459184-Gene Silencing, pubmed-meshheading:15459184-Genomic Imprinting, pubmed-meshheading:15459184-Humans, pubmed-meshheading:15459184-KCNQ Potassium Channels, pubmed-meshheading:15459184-KCNQ1 Potassium Channel, pubmed-meshheading:15459184-Membrane Proteins, pubmed-meshheading:15459184-Mice, pubmed-meshheading:15459184-Potassium Channels, Voltage-Gated, pubmed-meshheading:15459184-Promoter Regions, Genetic, pubmed-meshheading:15459184-Recombinant Proteins, pubmed-meshheading:15459184-Sequence Deletion
pubmed:year	2004
pubmed:articleTitle	NF-Y regulates the antisense promoter, bidirectional silencing, and differential epigenetic marks of the Kcnq1 imprinting control region.
pubmed:affiliation	Department of Development and Genetics, Evolutionary Biology Centrum, Norbyvägen 18A, Uppsala SE-75236 University, Uppsala, Sweden.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't