Linked Life Data

15457345

Source:http://linkedlifedata.com/resource/pubmed/id/15457345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-9-30
pubmed:abstractText
Studies in mouse and human have established that the exon encoding the neuroendocrine secretory protein Nesp55 is transcribed exclusively from the maternal allele. To study the imprinting status of Nesp55 in cattle, we examined transcripts of this exon in a wide range of tissues from four fetuses and the dam of one of the fetuses (not all tissues were examined in all subjects). A polymorphic site was used to determine transcript status as monoallelic or biallelic. The expression analysis was by direct sequencing of RT-PCR products amplified from the tissues. Nesp55 transcripts were detected in many tissues, in contrast to previous studies in which Nesp55 was detected by immunoassays in endocrine and nervous tissues only. In all five individuals, Nesp55 showed monoallelic expression. In one of the fetuses, parental origin of the expressed allele could not be determined; in the others monoallelic expression was clearly maternal. Thus, bovine Nesp55 is maternally expressed like its counterparts in mouse and human.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0938-8990
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
663-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Imprinting of Nesp55 gene in cattle.
pubmed:affiliation
Department of Dairy Science, University of Wisconsin, 1675 Observatory Drive, Madison 53706, USA. hkhatib@wisc.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't