Linked Life Data

15456723

Source:http://linkedlifedata.com/resource/pubmed/id/15456723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2004-10-15
pubmed:abstractText
In Arabidopsis, the EMBYRONIC FLOWER2 (EMF2), VERNALISATION2 (VRN2) and FERTILISATION INDEPENDENT ENDOSPERM2 (FIS2) genes encode related Polycomb-group (Pc-G) proteins. Their homologues in animals act together with other Pc-G proteins as part of a multimeric complex, Polycomb Repressive Complex 2 (PRC2), which functions as a histone methyltransferase. Despite similarities between the fis2 mutant phenotype and those of some other plant Pc-G members, it has remained unclear how the FIS2/EMF2/VRN2 class Pc-G genes interact with the others. We have identified a weak emf2 allele that reveals a novel phenotype with striking similarity to that of severe mutations in another Pc-G gene, CURLY LEAF (CLF), suggesting that the two genes may act in a common pathway. Consistent with this, we demonstrate that EMF2 and CLF interact genetically and that this reflects interaction of their protein products through two conserved motifs, the VEFS domain and the C5 domain. We show that the full function of CLF is masked by partial redundancy with a closely related gene, SWINGER (SWN), so that null clf mutants have a much less severe phenotype than emf2 mutants. Analysis in yeast further indicates a potential for the CLF and SWN proteins to interact with the other VEFS domain proteins VRN2 and FIS2. The functions of individual Pc-G members may therefore be broader than single mutant phenotypes reveal. We suggest that plants have Pc-G protein complexes similar to the Polycomb Repressive Complex2 (PRC2) of animals, but the duplication and subsequent diversification of components has given rise to different complexes with partially discrete functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5263-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15456723-Animals, pubmed-meshheading:15456723-Arabidopsis, pubmed-meshheading:15456723-Arabidopsis Proteins, pubmed-meshheading:15456723-Binding Sites, pubmed-meshheading:15456723-Flowers, pubmed-meshheading:15456723-Gene Expression Regulation, Plant, pubmed-meshheading:15456723-Genes, Plant, pubmed-meshheading:15456723-Homeodomain Proteins, pubmed-meshheading:15456723-Microscopy, Electron, Scanning, pubmed-meshheading:15456723-Multiprotein Complexes, pubmed-meshheading:15456723-Mutation, pubmed-meshheading:15456723-Phenotype, pubmed-meshheading:15456723-Phylogeny, pubmed-meshheading:15456723-Plant Leaves, pubmed-meshheading:15456723-Protein Binding, pubmed-meshheading:15456723-Protein Structure, Tertiary, pubmed-meshheading:15456723-Repressor Proteins, pubmed-meshheading:15456723-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
Interaction of Polycomb-group proteins controlling flowering in Arabidopsis.
pubmed:affiliation
Institute of Molecular Plant Science, School of Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JH, UK.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't