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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2004-9-29
pubmed:abstractText
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) for naive T cells and play an important role in cancer immunology. All-trans retinoic acid (ATRA) is known to be a differentiating agent in the treatment of acute promyelocytic leukemia (APL). In this study, we investigated whether ATRA can differentiate the retinoic acid (RA)-sensitive promyelocytic leukemic cell line, NB4, to DC-like cells and whether these differentiated cells can activate T cells. NB4 cells were differentiated to myeloid cells by 4, 6, and 8 days of ATRA treatment. NB4 cells up-regulated markers found in DCs, including HLA-DR, costimulatory molecules (CD80 and CD86), adhesion molecules (CD40), and chemokine receptors (CCR6) when cultured for 8 days in the presence of 1 microM ATRA. Upregulation of CD83 was also detected on the surface of ATRA-treated NB4 cells versus untreated cells. The addition of cytokines alone, such as GM-CSF or CD40 ligand, did not affect the expression of CD83 in untreated NB4 cells but they up-regulated CD83 in ATRA-treated cells. CD11b was coexpressed with CD80, CD83, and CD86 in ATRA-treated NB4 cells. In a functional assay, ATRA-treated NB4 cells stimulated T cell proliferation when challenged with Staphylococcus enterotoxin B. These results suggest that the differentiation of NB4 cells by ATRA causes the cells to express DC markers, and that ATRA-differentiated NB4 cells are able to present antigens to T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1567-5769
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1587-601
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15454112-Antigen-Presenting Cells, pubmed-meshheading:15454112-Antigens, CD, pubmed-meshheading:15454112-Antigens, CD11b, pubmed-meshheading:15454112-Antigens, CD27, pubmed-meshheading:15454112-Antigens, CD80, pubmed-meshheading:15454112-Antigens, CD86, pubmed-meshheading:15454112-Antigens, Surface, pubmed-meshheading:15454112-Blotting, Western, pubmed-meshheading:15454112-Cell Line, Tumor, pubmed-meshheading:15454112-Cell Proliferation, pubmed-meshheading:15454112-Cytokines, pubmed-meshheading:15454112-Dendritic Cells, pubmed-meshheading:15454112-Flow Cytometry, pubmed-meshheading:15454112-Humans, pubmed-meshheading:15454112-Korea, pubmed-meshheading:15454112-Leukemia, Promyelocytic, Acute, pubmed-meshheading:15454112-Lymphocyte Activation, pubmed-meshheading:15454112-Membrane Glycoproteins, pubmed-meshheading:15454112-Phagocytosis, pubmed-meshheading:15454112-RNA, Messenger, pubmed-meshheading:15454112-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15454112-T-Lymphocytes, pubmed-meshheading:15454112-Tretinoin, pubmed-meshheading:15454112-Up-Regulation
pubmed:year
2004
pubmed:articleTitle
Differential expression of dendritic cell markers by all-trans retinoic acid on human acute promyelocytic leukemic cell line.
pubmed:affiliation
Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong-A University, Busan 602-714, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't