pubmed-article:154510 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:154510 | lifeskim:mentions | umls-concept:C0027923 | lld:lifeskim |
pubmed-article:154510 | lifeskim:mentions | umls-concept:C0038615 | lld:lifeskim |
pubmed-article:154510 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
pubmed-article:154510 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
pubmed-article:154510 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:154510 | pubmed:dateCreated | 1979-5-23 | lld:pubmed |
pubmed-article:154510 | pubmed:abstractText | A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinate and phenazine methosulfate. Wild-type colonies reduced the dye and turned blue, whereas mutant colonies remained colorless. In this communication we present studies of a mutant, SDH-1, isolated by this method. The mutant had 18% of the succinic dehydrogenase activity of the parent strain used in the mutation experiments as determined from the ratio of Vmax activities obtained from Lineweaver-Burk plots. The SDH-1 mutant segregated in a Mendelian manner when back-crossed to its parent strain. Succinate oxidase activity in SDH-1 was low and was markedly inhibited by adenosine 5'-diphosphate. The succinate oxidase activity of the parent strain was high and was not affected by the presence of adenosine 5'-diphosphate. | lld:pubmed |
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pubmed-article:154510 | pubmed:language | eng | lld:pubmed |
pubmed-article:154510 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:154510 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:154510 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:154510 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:154510 | pubmed:month | Feb | lld:pubmed |
pubmed-article:154510 | pubmed:issn | 0021-9193 | lld:pubmed |
pubmed-article:154510 | pubmed:author | pubmed-author:EdwardsD LDL | lld:pubmed |
pubmed-article:154510 | pubmed:author | pubmed-author:UngerB WBW | lld:pubmed |
pubmed-article:154510 | pubmed:author | pubmed-author:BelsoleD MDM | lld:pubmed |
pubmed-article:154510 | pubmed:author | pubmed-author:GuzikH JHJ | lld:pubmed |
pubmed-article:154510 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:154510 | pubmed:volume | 137 | lld:pubmed |
pubmed-article:154510 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:154510 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:154510 | pubmed:pagination | 900-4 | lld:pubmed |
pubmed-article:154510 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:154510 | pubmed:year | 1979 | lld:pubmed |
pubmed-article:154510 | pubmed:articleTitle | Selection of succinic dehydrogenase mutants of Neurospora crassa. | lld:pubmed |
pubmed-article:154510 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:154510 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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