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pubmed-article:154510pubmed:abstractTextA method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinate and phenazine methosulfate. Wild-type colonies reduced the dye and turned blue, whereas mutant colonies remained colorless. In this communication we present studies of a mutant, SDH-1, isolated by this method. The mutant had 18% of the succinic dehydrogenase activity of the parent strain used in the mutation experiments as determined from the ratio of Vmax activities obtained from Lineweaver-Burk plots. The SDH-1 mutant segregated in a Mendelian manner when back-crossed to its parent strain. Succinate oxidase activity in SDH-1 was low and was markedly inhibited by adenosine 5'-diphosphate. The succinate oxidase activity of the parent strain was high and was not affected by the presence of adenosine 5'-diphosphate.lld:pubmed
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pubmed-article:154510pubmed:authorpubmed-author:EdwardsD LDLlld:pubmed
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pubmed-article:154510pubmed:authorpubmed-author:GuzikH JHJlld:pubmed
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pubmed-article:154510pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:154510pubmed:articleTitleSelection of succinic dehydrogenase mutants of Neurospora crassa.lld:pubmed
pubmed-article:154510pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:154510pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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