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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-5-23
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pubmed:abstractText |
A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinate and phenazine methosulfate. Wild-type colonies reduced the dye and turned blue, whereas mutant colonies remained colorless. In this communication we present studies of a mutant, SDH-1, isolated by this method. The mutant had 18% of the succinic dehydrogenase activity of the parent strain used in the mutation experiments as determined from the ratio of Vmax activities obtained from Lineweaver-Burk plots. The SDH-1 mutant segregated in a Mendelian manner when back-crossed to its parent strain. Succinate oxidase activity in SDH-1 was low and was markedly inhibited by adenosine 5'-diphosphate. The succinate oxidase activity of the parent strain was high and was not affected by the presence of adenosine 5'-diphosphate.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-147864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-149126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-234433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-406851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-4275427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-4310092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-4326769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-4349028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-5140191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/154510-558831
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
900-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Selection of succinic dehydrogenase mutants of Neurospora crassa.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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