Linked Life Data

15450754

Source:http://linkedlifedata.com/resource/pubmed/id/15450754

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-9-28
pubmed:abstractText
Stromal cell derived factor-1, SDF-1, belongs to the CXC family of chemokines and has been identified in mammals, amphibians, and fish. This chemokine has a diverse array of functions in organogenesis, hematopoeisis, B cell development and recruitment of immune system cells. Here, we report the cloning of the chicken SDF-1 ortholog and examine its temporal and spatial expression. The chicken SDF-1 cDNA contained an open reading frame encoding a predicted protein of 89 amino acids, which shared 40-75% identity to SDF-1 protein in other species. Protein folding simulation predicted a tertiary structure very similar to that obtained for human SDF-1. Recombinant chicken SDF-1 was produced using a prokaryotic expression system and the recombinant protein was shown to be biologically active in a calcium flux assay. The SDF-1 gene was found to be expressed ubiquitously and constitutively in adult tissues and was present as early as the primitive streak stage of chicken embryos.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0145-305X
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
143-52
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Cloning and characterization of chicken stromal cell derived factor-1.
pubmed:affiliation
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont., Canada N1G 2W1.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't