Linked Life Data

1542010

Source:http://linkedlifedata.com/resource/pubmed/id/1542010

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-4-8
pubmed:abstractText
Fed and fasted (18 h) adult male rats received a primed constant infusion of [3H]leucine for 15 to 180 min. Prosucrase-isomaltase (pro-SI) and sucrase were isolated from mixed jejunal mucosal membranes by immunoprecipitation and separated from one another by polyacrylamide gel electrophoresis under denaturing and reducing conditions. The rate at which pro-SI was processed to sucrase was calculated on the assumption that the steady-state specific radioactivity of leucine in pro-SI defined the pool of amino acids used in the formation of brush border sucrase. At isotopic steady state, pro-SI achieved a specific radioactivity that was higher than that of mucosal free leucine in both feeding groups. The relationship between the isotopic equilibrium of the free amino acid pools and pro-SI was sensitive to feeding status; the specific radioactivity of pro-SI was 25 and 55% (P less than 0.05) of the blood specific radioactivity in fed and fasted animals, respectively. The fractional rate of pro-SI processing tended to be higher (P less than 0.07) in fed (407%/d) than in fasted animals (274%/d). We conclude that the general mucosal free amino acid pool is not the amino acid pool from which pro-SI is synthesized and that the rate of pro-SI processing from the endoplasmic reticulum-Golgi membranes to the brush border membrane is sensitive to the feeding status of the animal.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3166
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
528-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Feeding status affects in vivo prosucrase.isomaltase processing in rat jejunum.
pubmed:affiliation
Department of Pediatrics, Baylor College of Medicine, Houston, TX.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.