Source:http://linkedlifedata.com/resource/pubmed/id/15384978
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001675,
umls-concept:C0023418,
umls-concept:C0023981,
umls-concept:C0030705,
umls-concept:C0040715,
umls-concept:C0185125,
umls-concept:C0205415,
umls-concept:C0337112,
umls-concept:C0443286,
umls-concept:C1510438,
umls-concept:C1511790,
umls-concept:C1524075,
umls-concept:C1555029,
umls-concept:C2603343
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| pubmed:issue |
1
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| pubmed:dateCreated |
2004-9-23
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| pubmed:abstractText |
The upfront application of molecular methods for identifying the fusion transcripts arising from balanced translocations in haematopoietic malignancies has several advantages: sensitivity is independent of its frequency, i.e. rare ones are not missed, cytogenetically cryptic aberrations are identified and it provides a platform for minimal residual disease (MRD) detection. Employing a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay identifying 27 fusion transcripts we prospectively analysed blood and/or bone marrow samples from 390 patients referred for diagnosis and treatment for acute leukaemia and chronic myeloproliferative disorders (CMPD) from a geographically well-defined region in Denmark. A total of 233 patients were diagnosed with acute myeloid leukaemia (AML), 95 with acute lymphoblastic leukaemia (ALL) origin and 62 patients were recorded as CMPD. Twenty-three percent AML, 32% ALL and 55% CMPD patients exhibited chromosomal aberrations detected by the multiplex RT-PCR. Cytogenetically cryptic translocations were seen in 15% of the cases. Conversely, the cytogenetic analysis identified chromosomal aberrations other than translocations in 45% of AML cases and 63% of ALL cases. We conclude that, while the fraction of translocation positive leukaemia patients in an unselected cohort is lower than hitherto believed, a molecular approach to their diagnosis is worthwhile, partly for identifying cryptic and rare translocations, partly for monitoring MRD.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0007-1048
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2004 Blackwell Publishing Ltd
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| pubmed:issnType |
Print
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| pubmed:volume |
127
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
59-66
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15384978-Acute Disease,
pubmed-meshheading:15384978-Adolescent,
pubmed-meshheading:15384978-Adult,
pubmed-meshheading:15384978-Aged,
pubmed-meshheading:15384978-Aged, 80 and over,
pubmed-meshheading:15384978-Child,
pubmed-meshheading:15384978-Child, Preschool,
pubmed-meshheading:15384978-Chromosome Aberrations,
pubmed-meshheading:15384978-Diagnosis, Differential,
pubmed-meshheading:15384978-Humans,
pubmed-meshheading:15384978-Infant,
pubmed-meshheading:15384978-Infant, Newborn,
pubmed-meshheading:15384978-Leukemia,
pubmed-meshheading:15384978-Leukemia, Myeloid,
pubmed-meshheading:15384978-Middle Aged,
pubmed-meshheading:15384978-Myeloproliferative Disorders,
pubmed-meshheading:15384978-Neoplasm, Residual,
pubmed-meshheading:15384978-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:15384978-Prospective Studies,
pubmed-meshheading:15384978-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15384978-Translocation, Genetic
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| pubmed:year |
2004
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| pubmed:articleTitle |
Prospective application of a multiplex reverse transcription-polymerase chain reaction assay for the detection of balanced translocations in leukaemia: a single-laboratory study of 390 paediatric and adult patients.
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| pubmed:affiliation |
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark.
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| pubmed:publicationType |
Journal Article
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