Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2004-11-3
pubmed:abstractText
The Ets-1 transcription factor is essential for normal development of the natural killer and T cell lineages; however, its role in B cell development remains poorly understood. To address this issue, we used gene targeting to inactivate Ets-1 in mice (Ets-1(-/-)). We show here that the development of B cell precursors, particularly steps requiring pre-B cell receptor function, is defective in Ets-1(-/-) mice. Peripheral B cell subsets were analyzed in RAG2-deficient mice reconstituted with Ets-1(-/-) fetal liver cells. In such Ets-1(-/-) chimeric mice, B cell precursors develop into IgM/IgD-bearing cells, but B-1a cells as well as transitional-2 and marginal zone B cell subsets of the spleen are absent. In response to B cell receptor stimulation, Ets-1(-/-) splenic B cells fail to express the CD69 and CD25 activation markers. Furthermore, despite activation of ERK and JNK signaling pathways, Ets-1-deficient B cells do not proliferate and die following BCR engagement. These findings demonstrate that the effect of Ets-1 inactivation is not restricted to the terminal B cell differentiation stage, but also affects the development and function of earlier B cell subsets.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3187-96
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15384043-Animals, pubmed-meshheading:15384043-Antigens, CD, pubmed-meshheading:15384043-B-Lymphocyte Subsets, pubmed-meshheading:15384043-B-Lymphocytes, pubmed-meshheading:15384043-Cell Differentiation, pubmed-meshheading:15384043-Flow Cytometry, pubmed-meshheading:15384043-Immunoglobulin Heavy Chains, pubmed-meshheading:15384043-Mice, pubmed-meshheading:15384043-Mice, Inbred C57BL, pubmed-meshheading:15384043-Mice, Knockout, pubmed-meshheading:15384043-Peritoneum, pubmed-meshheading:15384043-Proto-Oncogene Protein c-ets-1, pubmed-meshheading:15384043-Proto-Oncogene Proteins, pubmed-meshheading:15384043-Proto-Oncogene Proteins c-ets, pubmed-meshheading:15384043-Receptors, Antigen, B-Cell, pubmed-meshheading:15384043-Recombination, Genetic, pubmed-meshheading:15384043-Spleen, pubmed-meshheading:15384043-Transcription, Genetic, pubmed-meshheading:15384043-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
The development of early and mature B cells is impaired in mice deficient for the Ets-1 transcription factor.
pubmed:affiliation
INSERM Unité 462, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't