Linked Life Data

15383299

Source:http://linkedlifedata.com/resource/pubmed/id/15383299

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-9-22
pubmed:abstractText
The small nuclear RNA (snRNA)-activating protein complex (SNAP(c)) is a multi-subunit transcription factor characterized in humans and Drosophila melanogaster. It binds to an upstream sequence element (USE) of snRNA gene promoters and activates both RNA polymerase (pol) II and III-mediated transcription of snRNA genes. The first identified and partially characterized transcription factor in a trypanosomatid organism appears to be a SNAP(c) homologue. It was identified in Leptomonas seymouri and shown to specifically interact with the USE of the RNA pol II-transcribed spliced leader (SL) RNA gene. Recently, chromatin immunoprecipitation and a gel shift assay suggested that L. seymouri SNAP(c) also interacts with RNA pol III-transcribed U2 and U6 snRNA genes. Previously, we have characterized and epitope-tagged the Trypanosoma brucei homologue of the SNAP50 subunit. Here, we show by in vitro transcription competition and promoter pull-down assays that TbSNAP50 binds to the SL RNA gene promoter and parasite-specifically to the ribosomal RNA gene promoter. Conversely, we did not detect binding of the factor to U2 and U6 snRNA gene sequences. Since U snRNA gene promoters are structurally conserved among trypanosomatids, our findings contrast those obtained in L. seymouri and suggest that trypanosomatid SNAP(c) is not involved in RNA pol III-mediated transcription of U snRNA genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
293-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Failure to detect binding of Trypanosoma brucei SNAPc to U2 and U6 snRNA gene sequences by in vitro transcription competition and pull-down assays.
pubmed:affiliation
Center for Microbial Pathogenesis, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3710, USA.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't