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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-9-21
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pubmed:abstractText |
A polyepitope DNA vaccine has the potential to generate protective immune responses to a range of antigens in a single construct. We investigated whether it was possible to obtain responses to individual epitopes from different antigens, directly linked in a string, and whether the response to a given epitope was enhanced by adjacent epitopes within the construct. A polyepitope plasmid was created, which included three Th epitopes (influenza haemagglutinin, moth cytochrome c and ovalbumin), a Tc epitope (ovalbumin) and two B cell epitopes (haemagglutinin and ovalbumin). Mice were immunized with DNA by using a gene gun. Responses to the polyepitope DNA vaccine were compared with those to DNA vaccine comprising only the haemagglutinin Th and B epitopes (HAT(h)B) or with responses to the recombinant protein. These experiments showed that the polyepitope DNA vaccine induced greater antigen-specific responses to HAT(h)B peptide than the HAT(h)B DNA vaccine. Antigen-specific in vivo cytotoxic responses following polyepitope DNA vaccination were also clearly demonstrable. We conclude that a 'naked DNA' polyepitope vaccine generates specific responses to constituent epitopes and that adjacent irrelevant epitopes may enhance these responses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/H-2Kb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinin Glycoproteins...,
http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
363-71
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15379861-Animals,
pubmed-meshheading:15379861-Base Sequence,
pubmed-meshheading:15379861-Biolistics,
pubmed-meshheading:15379861-Chickens,
pubmed-meshheading:15379861-Cytochromes c,
pubmed-meshheading:15379861-Cytotoxicity, Immunologic,
pubmed-meshheading:15379861-DNA, Recombinant,
pubmed-meshheading:15379861-Epitopes,
pubmed-meshheading:15379861-Female,
pubmed-meshheading:15379861-H-2 Antigens,
pubmed-meshheading:15379861-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:15379861-Influenza Vaccines,
pubmed-meshheading:15379861-Interleukin-5,
pubmed-meshheading:15379861-Male,
pubmed-meshheading:15379861-Mice,
pubmed-meshheading:15379861-Mice, Inbred BALB C,
pubmed-meshheading:15379861-Mice, Inbred C57BL,
pubmed-meshheading:15379861-Molecular Sequence Data,
pubmed-meshheading:15379861-Moths,
pubmed-meshheading:15379861-Ovalbumin,
pubmed-meshheading:15379861-Plasmids,
pubmed-meshheading:15379861-Recombinant Proteins,
pubmed-meshheading:15379861-T-Lymphocyte Subsets,
pubmed-meshheading:15379861-Vaccines, DNA
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pubmed:year |
2004
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pubmed:articleTitle |
Bystander help within a polyepitope DNA vaccine improves immune responses to influenza antigens.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand. margaret.baird@stonebow.otago.ac.nz
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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