Linked Life Data

15378741

Source:http://linkedlifedata.com/resource/pubmed/id/15378741

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-10-14
pubmed:abstractText
Neisseria meningitidis is an encapsulated Gram-negative bacterium responsible for significant morbidity and mortality worldwide. Meningococci are opportunistic pathogens, carried in the nasopharynx of approximately 10% of asymptomatic adults. Occasionally they enter the bloodstream to cause septicaemia and meningitis. Meningococci are classified into serogroups on the basis of polysaccharide capsule diversity, and serogroup A strains have caused major epidemics mainly in the developing world. Here we describe a two-dimensional gel electrophoresis protein map of the serogroup A strain Z4970, a clinical isolate classified as ancestral to several pandemic waves. To our knowledge this is the first systematically annotated proteomic map for N. meningitidis. Total protein samples from bacteria grown on GC-agar were electrophoretically separated and protein species were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. We identified the products of 273 genes, covering several functional classes, including 94 proteins so far considered as hypothetical. We also describe several protein species encoded by genes reported by DNA microarray studies as being regulated in physiological conditions which are relevant to natural meningococcal pathogenicity. Since menA differs from other serogroups by having a fairly stable clonal population structure (i.e. with a low degree of variability), we envisaged comparative mapping as a useful tool for microevolution studies, in conjunction with established genotyping methods. As a proof of principle, we performed a comparative analysis on the B subunit of the meningococcal transferrin receptor, a vaccine candidate encoded by the tbpB gene, and a known marker of population diversity in meningococci. The results show that TbpB spot pattern variation observed in the maps of nine clinical isolates from diverse epidemic spreads, fits previous analyses based on allelic variations of the tbpB gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1615-9853
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2893-926
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15378741-Agar, pubmed-meshheading:15378741-Alleles, pubmed-meshheading:15378741-Bacterial Proteins, pubmed-meshheading:15378741-Densitometry, pubmed-meshheading:15378741-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:15378741-Genes, Bacterial, pubmed-meshheading:15378741-Genetic Variation, pubmed-meshheading:15378741-Humans, pubmed-meshheading:15378741-Hydrogen-Ion Concentration, pubmed-meshheading:15378741-Meningococcal Infections, pubmed-meshheading:15378741-Neisseria meningitidis, pubmed-meshheading:15378741-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:15378741-Proteome, pubmed-meshheading:15378741-Proteomics, pubmed-meshheading:15378741-Species Specificity, pubmed-meshheading:15378741-Spectrometry, Mass, Matrix-Assisted Laser..., pubmed-meshheading:15378741-Statistics as Topic
pubmed:year
2004
pubmed:articleTitle
Proteome analysis of Neisseria meningitidis serogroup A.
pubmed:affiliation
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Siena, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't