Source:http://linkedlifedata.com/resource/pubmed/id/15374630
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-9-17
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| pubmed:abstractText |
Efficacy of a safe and clinically utilized polyethylene glycol formulation (PEG-3350) to suppress intestinal tumors was investigated in the Apc(min) mouse-model of experimental carcinogenesis. Furthermore, based on our previous finding on the induction of apoptosis in HT-29 cells by PEG, we evaluated its ability to stimulate epithelial cell apoptosis in both Apc(min) mouse as well as AOM-treated rat as a potential molecular mechanism of chemoprevention. Twenty-two Apc(min) mice were randomized equally to PEG or vehicle (control) supplementation. Tumors were scored and uninvolved intestinal mucosal apoptosis was assayed using a modified terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) assay and by immunohistochemical detection of cleaved caspase-3. Supplementation of Apc(min) mice with 10% PEG 3350 (in drinking water) resulted in a 48% (P<0.05) reduction in intestinal tumor burden and induced 2-3 fold increase in mucosal apoptosis. Dietary supplementation of polyethylene glycol (5%) also stimulated colonic mucosal apoptosis 4-5 fold in AOM-treated rats, the regimen that we previously reported to reduce tumor burden by 76% (P<0.05). In summary, we demonstrate, for the first time, that PEG does protect against Apc(min) mouse tumorigenesis. The correlation between pro-apoptotic actions and chemopreventive efficacy of PEG in these models strongly implicates induction of apoptosis as one of the impending mechanisms of chemoprevention.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Azoxymethane,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Surface-Active Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Water
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0304-3835
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
8
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| pubmed:volume |
215
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
35-42
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15374630-Animals,
pubmed-meshheading:15374630-Apoptosis,
pubmed-meshheading:15374630-Azoxymethane,
pubmed-meshheading:15374630-Carcinogens,
pubmed-meshheading:15374630-Caspase 3,
pubmed-meshheading:15374630-Caspases,
pubmed-meshheading:15374630-Dietary Supplements,
pubmed-meshheading:15374630-Epithelial Cells,
pubmed-meshheading:15374630-Genes, APC,
pubmed-meshheading:15374630-Humans,
pubmed-meshheading:15374630-In Situ Nick-End Labeling,
pubmed-meshheading:15374630-Intestinal Mucosa,
pubmed-meshheading:15374630-Intestinal Neoplasms,
pubmed-meshheading:15374630-Male,
pubmed-meshheading:15374630-Mice,
pubmed-meshheading:15374630-Mice, Knockout,
pubmed-meshheading:15374630-Polyethylene Glycols,
pubmed-meshheading:15374630-Rats,
pubmed-meshheading:15374630-Rats, Inbred F344,
pubmed-meshheading:15374630-Surface-Active Agents,
pubmed-meshheading:15374630-Water
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| pubmed:year |
2004
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| pubmed:articleTitle |
Polyethylene glycol inhibits intestinal neoplasia and induces epithelial apoptosis in Apc(min) mice.
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| pubmed:affiliation |
Department of Medicine, Evanston-Northwestern Healthcare Research Institute, 1001 University Place, Evanston, IL 60201, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study
|