Linked Life Data

1537294

Source:http://linkedlifedata.com/resource/pubmed/id/1537294

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-4-2
pubmed:abstractText
To examine the developmental expression and regulation of P450SCC and P450(17 alpha) in the rat placenta, trophoblast and decidual tissue were removed by dissection from conceptuses obtained from rats on selected days of pregnancy. Total cellular and poly(A)+ RNA and microsomal and mitochondrial fractions were isolated and analyzed for the presence of P450(17) alpha and P450SCC messenger RNA (mRNA) and protein by Northern and Western blot analysis. P450(17) alpha and P450SCC mRNA were detected in the trophoblast but not in the decidual tissue. Western blot studies demonstrated that the immunoreactive P450(17) alpha in the rat placenta is a 79-kilodalton protein, having a slower mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis than has been reported for other tissue. Antiserum preabsorbed with pure P450(17) alpha was unable to detect this protein, and immunoprecipitation indicated that it is associated with enzyme activity. Development studies have revealed that the two steroidogenic enzymes are differentially expressed during the progression of pregnancy. Whereas P450SCC mRNA and protein increase abruptly between days 10-12 of pregnancy, decline thereafter, and remain low, those of P450(17) alpha increase slowly and progressively, peaking on day 18 and declining just before parturition. It is the changes in P450(17) alpha and not that of P450SCC which appear to be intimately linked to the previously reported changes in placental production of androgen. To examine whether P450(17) alpha and/or P450SCC became expressed from midpregnancy because of the rapid decline in LH that occurs at this stage, pregnant rats were treated with low but sustained levels of human CG (hCG) in order to prevent the drop in LH activity. hCG treatment caused a remarkable down regulation in the expression of both P450SCC and P450(17) alpha message and protein. In summary, the results of this investigation have established, for the first time, the presence of messages for both P450(17) alpha and P450SCC in the trophoblast tissue forming the rat placenta. The results have revealed that these two enzymes are differentially expressed during the progression of pregnancy and that the expression of their genes is down-regulated by LH/hCG.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:geneSymbol
P450<down>17&agr;</down>, P450<down>SCC</down>
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1309-17
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1537294-Androgens, pubmed-meshheading:1537294-Animals, pubmed-meshheading:1537294-Blotting, Northern, pubmed-meshheading:1537294-Blotting, Western, pubmed-meshheading:1537294-Cholesterol Side-Chain Cleavage Enzyme, pubmed-meshheading:1537294-Chorionic Gonadotropin, pubmed-meshheading:1537294-Down-Regulation, pubmed-meshheading:1537294-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:1537294-Female, pubmed-meshheading:1537294-Gene Expression Regulation, Enzymologic, pubmed-meshheading:1537294-Immunosorbent Techniques, pubmed-meshheading:1537294-Luteinizing Hormone, pubmed-meshheading:1537294-Placenta, pubmed-meshheading:1537294-Precipitin Tests, pubmed-meshheading:1537294-Pregnancy, pubmed-meshheading:1537294-RNA, Messenger, pubmed-meshheading:1537294-Rats, pubmed-meshheading:1537294-Steroid 17-alpha-Hydroxylase
pubmed:year
1992
pubmed:articleTitle
P450(17 alpha) and P450SCC gene expression and regulation in the rat placenta.
pubmed:affiliation
Department of Physiology and Biophysics, University of Illinois College of Medicine, Chicago 60612.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't