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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2004-9-10
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pubmed:abstractText |
The cytidine deaminase hAPOBEC3G is an antiviral human factor that counteracts the replication of HIV-1 in absence of the Vif protein. hAPOBEC3G is packaged into virus particles and lethally hypermutates HIV-1. In this work, we examine the mechanisms governing hAPOBEC3G packaging. By GST pull-down and co-immunoprecipitation assays, we show that hAPOBEC3G binds to HIV-1 Pr55 Gag and its NC domain and to the RT and IN domains contained in Pr160 Gag-Pol. We demonstrate that the expression of HIV-1 Gag is sufficient to induce the packaging of hAPOBEC3G into Gag particles. Gag-Pol polypeptides containing RT and IN domains, as well as HIV-1 genomic RNA, seem not to be necessary for hAPOBEC3G packaging. Lastly, we show that hAPOBEC3G and its murine ortholog are packaged into HIV-1 and MLV Gag particles. We conclude that the Gag polypeptides from distant retroviruses have conserved domains allowing the packaging of the host antiviral factor APOBEC3G.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/APOBEC3G protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytidine Deaminase,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoside Deaminases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/gag Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/p55 gag precursor protein, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/pol Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/pr160 gag-pol precursor protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
566-73
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15358144-Animals,
pubmed-meshheading:15358144-Cytidine Deaminase,
pubmed-meshheading:15358144-Gene Products, gag,
pubmed-meshheading:15358144-HIV-1,
pubmed-meshheading:15358144-HeLa Cells,
pubmed-meshheading:15358144-Humans,
pubmed-meshheading:15358144-Leukemia Virus, Murine,
pubmed-meshheading:15358144-Mice,
pubmed-meshheading:15358144-Nucleoside Deaminases,
pubmed-meshheading:15358144-Protein Isoforms,
pubmed-meshheading:15358144-Protein Precursors,
pubmed-meshheading:15358144-Protein Structure, Tertiary,
pubmed-meshheading:15358144-Proteins,
pubmed-meshheading:15358144-Recombinant Fusion Proteins,
pubmed-meshheading:15358144-Repressor Proteins,
pubmed-meshheading:15358144-Virion,
pubmed-meshheading:15358144-Virus Replication,
pubmed-meshheading:15358144-gag Gene Products, Human Immunodeficiency Virus,
pubmed-meshheading:15358144-pol Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2004
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pubmed:articleTitle |
HIV-1 and MLV Gag proteins are sufficient to recruit APOBEC3G into virus-like particles.
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pubmed:affiliation |
Inserm Unité 372, Université de la Méditerranée, 163 Avenue de Luminy, BP178, 13276 Marseille Cedex 09, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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