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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-9-10
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pubmed:abstractText |
Studies of SARS coronavirus (SARS-CoV)-the causative agent of severe acute respiratory syndrome (SARS)-have been hampered by its high transmission rate and the pathogenicity of this virus. To permit analysis of the host range and entry mechanism of SARS-CoV, we incorporated the humanized SARS-CoV spike (S) glycoprotein into HIV particles to generate a highly infectious SARS-CoV pseudotyped virus. The infection on Vero E6-a permissive cell line to SARS-CoV-could be neutralized by sera from convalescent SARS patients, and the entry was a pH-dependent process. With these highly infectious SARS-CoV pseudotypes, several cell lines derived from various tissues were revealed as susceptible to SARS-CoV, which were highly corresponding to the expression pattern of virus's receptor angiotensin-converting enzyme 2 (ACE2). In addition, we also demonstrated angiotensin 1 converting enzyme (ACE)-the homologue of ACE2 could not function as a receptor for SARS-CoV.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carboxypeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidyl-Dipeptidase A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/angiotensin converting enzyme 2,
http://linkedlifedata.com/resource/pubmed/chemical/spike glycoprotein, coronavirus
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:ChenJianJ,
pubmed-author:ChenJianguoJ,
pubmed-author:DengHongkuiH,
pubmed-author:DingMingxiaoM,
pubmed-author:HouJ PJP,
pubmed-author:NieYuchunY,
pubmed-author:PanZ RZR,
pubmed-author:QuXiuxiaX,
pubmed-author:ShiXuanlingX,
pubmed-author:SongXijunX,
pubmed-author:VighB JBJ,
pubmed-author:WangGuangwenG,
pubmed-author:WangPeigangP,
pubmed-author:WangZaiZ,
pubmed-author:YinXiaoleiX,
pubmed-author:ZhengAihuaA
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
994-1000
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15358126-Animals,
pubmed-meshheading:15358126-Base Sequence,
pubmed-meshheading:15358126-Carboxypeptidases,
pubmed-meshheading:15358126-Cell Line,
pubmed-meshheading:15358126-Cercopithecus aethiops,
pubmed-meshheading:15358126-Chimera,
pubmed-meshheading:15358126-DNA, Recombinant,
pubmed-meshheading:15358126-Gene Expression,
pubmed-meshheading:15358126-Genes, Viral,
pubmed-meshheading:15358126-HIV,
pubmed-meshheading:15358126-Humans,
pubmed-meshheading:15358126-Hydrogen-Ion Concentration,
pubmed-meshheading:15358126-Membrane Glycoproteins,
pubmed-meshheading:15358126-Mice,
pubmed-meshheading:15358126-Peptidyl-Dipeptidase A,
pubmed-meshheading:15358126-Receptors, Virus,
pubmed-meshheading:15358126-SARS Virus,
pubmed-meshheading:15358126-Vero Cells,
pubmed-meshheading:15358126-Viral Envelope Proteins,
pubmed-meshheading:15358126-Virulence,
pubmed-meshheading:15358126-Virus Assembly
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pubmed:year |
2004
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pubmed:articleTitle |
Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
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pubmed:affiliation |
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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