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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
47
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pubmed:dateCreated |
2004-11-15
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pubmed:abstractText |
Adenomatous polyposis coli (APC) protein and Axin form a complex that mediates the down-regulation of beta-catenin, a key effector of Wnt signaling. Truncation mutations in APC are responsible for familial and sporadic colorectal tumors due to failure in the down-regulation of beta-catenin. While the regulation of beta-catenin by APC has been extensively studied, the regulation of APC itself has received little attention. Here we show that the level of APC is down-regulated by the ubiquitin-proteasome pathway and that Wnt signaling inhibits the process. The domain responsible for the down-regulation and direct ubiquitination was identified. We also show an unexpected role for Axin in facilitating the ubiquitination-proteasome-mediated down-regulation of APC through the oligomerization of Axin. Our results suggest a new mechanism for the regulation of APC by Axin and Wnt signaling.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenomatous Polyposis Coli Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Axin Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/axin1 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/beta-catenin protein, Xenopus
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
49188-98
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15355978-Adenomatous Polyposis Coli Protein,
pubmed-meshheading:15355978-Amino Acids,
pubmed-meshheading:15355978-Animals,
pubmed-meshheading:15355978-Axin Protein,
pubmed-meshheading:15355978-Binding Sites,
pubmed-meshheading:15355978-Blotting, Western,
pubmed-meshheading:15355978-Cell Line,
pubmed-meshheading:15355978-Cell Nucleus,
pubmed-meshheading:15355978-Culture Media, Conditioned,
pubmed-meshheading:15355978-Cytoskeletal Proteins,
pubmed-meshheading:15355978-Down-Regulation,
pubmed-meshheading:15355978-Epitopes,
pubmed-meshheading:15355978-Gene Deletion,
pubmed-meshheading:15355978-Humans,
pubmed-meshheading:15355978-Immunoblotting,
pubmed-meshheading:15355978-Immunoprecipitation,
pubmed-meshheading:15355978-Microscopy, Fluorescence,
pubmed-meshheading:15355978-Plasmids,
pubmed-meshheading:15355978-Proteasome Endopeptidase Complex,
pubmed-meshheading:15355978-Protein Structure, Tertiary,
pubmed-meshheading:15355978-Proteins,
pubmed-meshheading:15355978-Proto-Oncogene Proteins,
pubmed-meshheading:15355978-Repressor Proteins,
pubmed-meshheading:15355978-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15355978-Signal Transduction,
pubmed-meshheading:15355978-Time Factors,
pubmed-meshheading:15355978-Trans-Activators,
pubmed-meshheading:15355978-Transfection,
pubmed-meshheading:15355978-Ubiquitin,
pubmed-meshheading:15355978-Up-Regulation,
pubmed-meshheading:15355978-Wnt Proteins,
pubmed-meshheading:15355978-Wnt3 Protein,
pubmed-meshheading:15355978-Xenopus,
pubmed-meshheading:15355978-Xenopus Proteins,
pubmed-meshheading:15355978-beta Catenin
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pubmed:year |
2004
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pubmed:articleTitle |
Adenomatous polyposis coli is down-regulated by the ubiquitin-proteasome pathway in a process facilitated by Axin.
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pubmed:affiliation |
Laboratory of Ophthalmology and Visual Science, The Catholic University of Korea, 505 Banpo-dong, Seocho-ku, Seoul, 137-701, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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