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rdf:type |
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lifeskim:mentions |
umls-concept:C0001675,
umls-concept:C0007589,
umls-concept:C0028944,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035647,
umls-concept:C0205409,
umls-concept:C0927232,
umls-concept:C1511938,
umls-concept:C1533691,
umls-concept:C1709634
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pubmed:issue |
6
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pubmed:dateCreated |
2004-9-9
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pubmed:abstractText |
There is a long-standing controversy as to whether oligodendrocytes may be capable of cell division and thus contribute to remyelination. We recently published evidence that a subpopulation of myelin oligodendrocyte glycoprotein (MOG)-expressing cells in the adult rat spinal cord co-expressed molecules previously considered to be restricted to oligodendrocyte progenitors [G. Li et al. (2002) Brain Pathol., 12, 463-471]. To further investigate the properties of MOG-expressing cells, anti-MOG-immunosorted cells were grown in culture and transplanted into acute demyelinating lesions. The immunosorting protocol yielded a cell preparation in which over 98% of the viable cells showed anti-MOG- and O1-immunoreactivity; 12-15% of the anti-MOG-immunosorted cells co-expressed platelet-derived growth factor alpha receptor (PDGFRalpha) or the A2B5-epitope. When cultured in serum-free medium containing EGF and FGF-2, 15-18% of the anti-MOG-immunosorted cells lost anti-MOG- and O1-immunoreactivity and underwent cell division. On removal of these growth factors, cells differentiated into oligodendrocytes, or astrocytes and Schwann cells when the differentiation medium contained BMPs. Transplantation of anti-MOG-immunosorted cells into areas of acute demyelination immediately after isolation resulted in the generation of remyelinating oligodendrocytes and Schwann cells. Our studies indicate that the adult rat CNS contains a significant number of oligodendrocyte precursors that express MOG and galactocerebroside, molecules previously considered restricted to mature oligodendrocytes. This may explain why myelin-bearing oligodendrocytes were considered capable of generating remyelinating cells. Our study also provides evidence that the adult oligodendrocyte progenitor can be considered as a source of the Schwann cells that remyelinate demyelinated CNS axons following concurrent destruction of oligodendrocytes and astrocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclophilins,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ethidium,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/HOE 33342,
http://linkedlifedata.com/resource/pubmed/chemical/Intermediate Filament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myelin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myelin-Associated Glycoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-6,
http://linkedlifedata.com/resource/pubmed/chemical/Pou3f1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Platelet-Derived Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/myelin oligodendrocyte glycoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/nestin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0953-816X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1445-60
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15355312-Animals,
pubmed-meshheading:15355312-Axons,
pubmed-meshheading:15355312-Benzimidazoles,
pubmed-meshheading:15355312-Blotting, Western,
pubmed-meshheading:15355312-Bone Morphogenetic Proteins,
pubmed-meshheading:15355312-Bromodeoxyuridine,
pubmed-meshheading:15355312-Cell Count,
pubmed-meshheading:15355312-Cell Differentiation,
pubmed-meshheading:15355312-Cell Division,
pubmed-meshheading:15355312-Cell Survival,
pubmed-meshheading:15355312-Cells, Cultured,
pubmed-meshheading:15355312-Central Nervous System,
pubmed-meshheading:15355312-Cyclophilins,
pubmed-meshheading:15355312-Demyelinating Diseases,
pubmed-meshheading:15355312-Epidermal Growth Factor,
pubmed-meshheading:15355312-Ethidium,
pubmed-meshheading:15355312-Female,
pubmed-meshheading:15355312-Flow Cytometry,
pubmed-meshheading:15355312-Glial Fibrillary Acidic Protein,
pubmed-meshheading:15355312-Immunohistochemistry,
pubmed-meshheading:15355312-Intermediate Filament Proteins,
pubmed-meshheading:15355312-Microscopy, Electron, Scanning Transmission,
pubmed-meshheading:15355312-Myelin Proteins,
pubmed-meshheading:15355312-Myelin Sheath,
pubmed-meshheading:15355312-Myelin-Associated Glycoprotein,
pubmed-meshheading:15355312-Nerve Regeneration,
pubmed-meshheading:15355312-Nerve Tissue Proteins,
pubmed-meshheading:15355312-Octamer Transcription Factor-6,
pubmed-meshheading:15355312-Oligodendroglia,
pubmed-meshheading:15355312-Rats,
pubmed-meshheading:15355312-Receptors, Platelet-Derived Growth Factor,
pubmed-meshheading:15355312-Stem Cells,
pubmed-meshheading:15355312-Time Factors,
pubmed-meshheading:15355312-Transcription Factors,
pubmed-meshheading:15355312-Transplants
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pubmed:year |
2004
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pubmed:articleTitle |
The remyelinating potential and in vitro differentiation of MOG-expressing oligodendrocyte precursors isolated from the adult rat CNS.
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pubmed:affiliation |
Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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