The complementary DNA for the feline cardiac sarcoplasmic reticulum (SR) Ca(2+)-ATPase has been cloned and sequenced. The deduced amino acid sequence consists of 997 amino acid residues which shows greater than 98% identity with the pig, rabbit and human SR Ca(2+)-ATPase. The 5' and 3' untranslated regions are also strikingly similar to the published rabbit sequence.