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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-9-7
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pubmed:abstractText |
Eph/ephrin receptors and ligands mediate cell-cell interaction through reciprocal signaling upon juxtacrine contact, and play a critical role in embryonic patterning, neuronal targeting, and vascular assembly. To study transmembrane ephrin-B ligand trafficking, we determined the cellular localization of ephrin-B1-GFP upon engagement by EphB1. Under normal culture conditions ephrin-B1-GFP is localized to the plasma membrane, mostly at the lateral cell borders. Addition of soluble EphB1-Fc receptor induces ephrin-B1-GFP clustering on the cell surface and subsequent internalization, as judged by biochemical studies, electron microscopy, and co-localization with endosomal markers. A dominant-negative mutant of dynamin or potassium depletion blocks ephrin-B1 endocytosis. These results suggest that ephrin-B1 internalization is an active receptor-mediated process that utilizes the clathrin-mediated endocytic pathway.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2004 Elsevier Inc.
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
323
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17-23
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15351694-Animals,
pubmed-meshheading:15351694-Biotinylation,
pubmed-meshheading:15351694-Blotting, Western,
pubmed-meshheading:15351694-CHO Cells,
pubmed-meshheading:15351694-Cell Membrane,
pubmed-meshheading:15351694-Cell Separation,
pubmed-meshheading:15351694-Cells, Cultured,
pubmed-meshheading:15351694-Clathrin,
pubmed-meshheading:15351694-Cricetinae,
pubmed-meshheading:15351694-DNA, Complementary,
pubmed-meshheading:15351694-Dynamins,
pubmed-meshheading:15351694-Endocytosis,
pubmed-meshheading:15351694-Ephrin-B1,
pubmed-meshheading:15351694-Flow Cytometry,
pubmed-meshheading:15351694-Genes, Dominant,
pubmed-meshheading:15351694-Genetic Vectors,
pubmed-meshheading:15351694-Humans,
pubmed-meshheading:15351694-Ligands,
pubmed-meshheading:15351694-Microscopy, Confocal,
pubmed-meshheading:15351694-Microscopy, Electron,
pubmed-meshheading:15351694-Mutation,
pubmed-meshheading:15351694-Potassium,
pubmed-meshheading:15351694-Receptors, Eph Family,
pubmed-meshheading:15351694-Signal Transduction,
pubmed-meshheading:15351694-Time Factors,
pubmed-meshheading:15351694-Transfection,
pubmed-meshheading:15351694-Umbilical Veins
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pubmed:year |
2004
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pubmed:articleTitle |
Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway.
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pubmed:affiliation |
Division of Rheumatology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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