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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-9-7
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pubmed:abstractText |
Neural stem cells (NSCs) are multipotential progenitor cells that have self-renewal activity. Since the fates of the NSCs in situ depend on their niche containing growth factors and cytokines, we performed surface enhanced laser desorption/ionization time-of flight mass spectrometry (SELDI-TOF-MS) to screen for differentially secreted proteins in conditioned medium of neural stem cells and compared with that of NIH3T3 cells. A 15.3-kDa protein detected only in the conditioned medium of neural stem cells was determined as pleiotrophin (PTN) by SELDI-TOF-MS and ProteinChip-tandem MS systems. Identification of pleiotrophin was further confirmed by one-dimensional SDS gel electrophoresis and Edman degradation analysis. The mRNA transcripts of PTN and its receptors [receptor protein tyrosine phosphatase (RPTP) beta/zeta, N-syndecan and anaplastic lymphoma kinase (ALK)] were detected in neurosphere, suggesting that pleiotrophin signaling systems are present in the neural stem cells and are involved in the modulation of fate of neural stem cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Ptprz1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-Like Protein Tyrosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Sdc3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecan-3,
http://linkedlifedata.com/resource/pubmed/chemical/anaplastic lymphoma kinase,
http://linkedlifedata.com/resource/pubmed/chemical/pleiotrophin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0165-3806
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-97
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15351507-Animals,
pubmed-meshheading:15351507-Carrier Proteins,
pubmed-meshheading:15351507-Cell Communication,
pubmed-meshheading:15351507-Cell Differentiation,
pubmed-meshheading:15351507-Cells, Cultured,
pubmed-meshheading:15351507-Culture Media, Conditioned,
pubmed-meshheading:15351507-Cytokines,
pubmed-meshheading:15351507-Mass Spectrometry,
pubmed-meshheading:15351507-Membrane Glycoproteins,
pubmed-meshheading:15351507-Mice,
pubmed-meshheading:15351507-Mice, Inbred ICR,
pubmed-meshheading:15351507-Multipotent Stem Cells,
pubmed-meshheading:15351507-NIH 3T3 Cells,
pubmed-meshheading:15351507-Nerve Tissue Proteins,
pubmed-meshheading:15351507-Neurons,
pubmed-meshheading:15351507-Protein Tyrosine Phosphatases,
pubmed-meshheading:15351507-Protein-Tyrosine Kinases,
pubmed-meshheading:15351507-Proteoglycans,
pubmed-meshheading:15351507-RNA, Messenger,
pubmed-meshheading:15351507-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:15351507-Receptor-Like Protein Tyrosine Phosphatases, Class 5,
pubmed-meshheading:15351507-Signal Transduction,
pubmed-meshheading:15351507-Spheroids, Cellular,
pubmed-meshheading:15351507-Syndecan-3
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pubmed:year |
2004
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pubmed:articleTitle |
Identification of pleiotrophin in conditioned medium secreted from neural stem cells by SELDI-TOF and SELDI-tandem mass spectrometry.
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pubmed:affiliation |
Department of Neurosurgery, Faculty of Medicine, Saga University, Japan.
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pubmed:publicationType |
Journal Article
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