Linked Life Data

15351484

Source:http://linkedlifedata.com/resource/pubmed/id/15351484

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-9-7
pubmed:abstractText
The cell cycle phase distributions of Hz-AM1 cells grown in monolayer culture were G1 = 49.7 +/- 3.3%, S = 22.7 +/- 3.8% and G2/M = 27.8 +/- 4.2% without >4N DNA content. The culture doubling time was about 40 h and the duration of the G1, S and G2/M phases was estimated to be 10, 14 and 16 h, respectively. HaSNPV infection of Hz-AM1 cells resulted in both unsynchronized and synchronized G1 phase arrest at G2/M phase. HaSNPV infection also resulted in the appearance of more than 4N DNA content, which accumulated to the highest levels 72 h post-infection. We also found that the expression level of cyclin B1 increased significantly after 16 h post-infection, while cyclin A did not show any change. This observation supports the Hz-AM1-infected arrest at the G2/M phage. Cytoplasm location of cyclin B1 indicated that the Hz-AM1 cell cycle arrest was at the G2 phase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-20
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Heliocoverpa armigera single nucleocapsid nucleopolyhedrovirus induces Hz-AM1 cell cycle arrest at the G2 phase with accumulation of cyclin B1.
pubmed:affiliation
Institution of Entomology, Central China Normal University, Wuhan 430070, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't