rdf:type |
|
lifeskim:mentions |
umls-concept:C0001041,
umls-concept:C0019564,
umls-concept:C0021549,
umls-concept:C0027882,
umls-concept:C0030685,
umls-concept:C0033268,
umls-concept:C0391871,
umls-concept:C0441655,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1963578
|
pubmed:issue |
47
|
pubmed:dateCreated |
2004-11-15
|
pubmed:abstractText |
Intracellular Ca2+ store release contributes to activity-dependent synaptic plasticity in the central nervous system by modulating the amplitude, propagation, and temporal dynamics of cytoplasmic Ca2+ changes. However, neuronal Ca2+ stores can be relatively insensitive to increases in the store-mobilizing messenger inositol 1,4,5-trisphosphate (IP3). Using a fluorescent biosensor we have visualized M1 muscarinic acetylcholine (mACh) receptor signaling in individual hippocampal neurons and observed increased IP3 production in the absence of concurrent Ca2+ store release. However, coincident glutamate-mediated synaptic activity elicited enhanced and oscillatory IP3 production that was dependent upon ongoing mACh receptor stimulation and S-alpha-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid receptor activation of Ca2+ entry. Moreover, the enhanced levels of IP3 now mobilized Ca2+ from intracellular stores that were refractory to the activation of mACh receptors alone. We conclude that convergent ionotropic and metabotropic receptor inputs can facilitate Ca2+ signaling by enhancing IP3 production as well as augmenting release by Ca2+-induced Ca2+ release.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Picrotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, AMPA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Amino-3-hydroxy-5-methyl-4-iso...
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pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0021-9258
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
19
|
pubmed:volume |
279
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
49036-44
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15342646-Animals,
pubmed-meshheading:15342646-Biosensing Techniques,
pubmed-meshheading:15342646-Calcium,
pubmed-meshheading:15342646-Cells, Cultured,
pubmed-meshheading:15342646-Dose-Response Relationship, Drug,
pubmed-meshheading:15342646-Electrophysiology,
pubmed-meshheading:15342646-Fluorescent Dyes,
pubmed-meshheading:15342646-Glutamic Acid,
pubmed-meshheading:15342646-Green Fluorescent Proteins,
pubmed-meshheading:15342646-Hippocampus,
pubmed-meshheading:15342646-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:15342646-Microscopy, Confocal,
pubmed-meshheading:15342646-Microscopy, Fluorescence,
pubmed-meshheading:15342646-Neurons,
pubmed-meshheading:15342646-Oscillometry,
pubmed-meshheading:15342646-Picrotoxin,
pubmed-meshheading:15342646-Plasmids,
pubmed-meshheading:15342646-Rats,
pubmed-meshheading:15342646-Receptors, AMPA,
pubmed-meshheading:15342646-Receptors, Muscarinic,
pubmed-meshheading:15342646-Synapses,
pubmed-meshheading:15342646-Time Factors,
pubmed-meshheading:15342646-Transfection,
pubmed-meshheading:15342646-alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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pubmed:year |
2004
|
pubmed:articleTitle |
Synaptic activity augments muscarinic acetylcholine receptor-stimulated inositol 1,4,5-trisphosphate production to facilitate Ca2+ release in hippocampal neurons.
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pubmed:affiliation |
Department of Cell Physiology & Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, Leicester, LE1 9HN, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|