Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
47
pubmed:dateCreated
2004-11-15
pubmed:abstractText
Intracellular Ca2+ store release contributes to activity-dependent synaptic plasticity in the central nervous system by modulating the amplitude, propagation, and temporal dynamics of cytoplasmic Ca2+ changes. However, neuronal Ca2+ stores can be relatively insensitive to increases in the store-mobilizing messenger inositol 1,4,5-trisphosphate (IP3). Using a fluorescent biosensor we have visualized M1 muscarinic acetylcholine (mACh) receptor signaling in individual hippocampal neurons and observed increased IP3 production in the absence of concurrent Ca2+ store release. However, coincident glutamate-mediated synaptic activity elicited enhanced and oscillatory IP3 production that was dependent upon ongoing mACh receptor stimulation and S-alpha-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid receptor activation of Ca2+ entry. Moreover, the enhanced levels of IP3 now mobilized Ca2+ from intracellular stores that were refractory to the activation of mACh receptors alone. We conclude that convergent ionotropic and metabotropic receptor inputs can facilitate Ca2+ signaling by enhancing IP3 production as well as augmenting release by Ca2+-induced Ca2+ release.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49036-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15342646-Animals, pubmed-meshheading:15342646-Biosensing Techniques, pubmed-meshheading:15342646-Calcium, pubmed-meshheading:15342646-Cells, Cultured, pubmed-meshheading:15342646-Dose-Response Relationship, Drug, pubmed-meshheading:15342646-Electrophysiology, pubmed-meshheading:15342646-Fluorescent Dyes, pubmed-meshheading:15342646-Glutamic Acid, pubmed-meshheading:15342646-Green Fluorescent Proteins, pubmed-meshheading:15342646-Hippocampus, pubmed-meshheading:15342646-Inositol 1,4,5-Trisphosphate, pubmed-meshheading:15342646-Microscopy, Confocal, pubmed-meshheading:15342646-Microscopy, Fluorescence, pubmed-meshheading:15342646-Neurons, pubmed-meshheading:15342646-Oscillometry, pubmed-meshheading:15342646-Picrotoxin, pubmed-meshheading:15342646-Plasmids, pubmed-meshheading:15342646-Rats, pubmed-meshheading:15342646-Receptors, AMPA, pubmed-meshheading:15342646-Receptors, Muscarinic, pubmed-meshheading:15342646-Synapses, pubmed-meshheading:15342646-Time Factors, pubmed-meshheading:15342646-Transfection, pubmed-meshheading:15342646-alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
pubmed:year
2004
pubmed:articleTitle
Synaptic activity augments muscarinic acetylcholine receptor-stimulated inositol 1,4,5-trisphosphate production to facilitate Ca2+ release in hippocampal neurons.
pubmed:affiliation
Department of Cell Physiology & Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, Leicester, LE1 9HN, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't