| pubmed-article:15342570 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15342570 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
| pubmed-article:15342570 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:15342570 | lifeskim:mentions | umls-concept:C1187839 | lld:lifeskim |
| pubmed-article:15342570 | lifeskim:mentions | umls-concept:C0031437 | lld:lifeskim |
| pubmed-article:15342570 | lifeskim:mentions | umls-concept:C1519717 | lld:lifeskim |
| pubmed-article:15342570 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:15342570 | pubmed:issue | 18 | lld:pubmed |
| pubmed-article:15342570 | pubmed:dateCreated | 2004-9-2 | lld:pubmed |
| pubmed-article:15342570 | pubmed:abstractText | Burkholderia cenocepacia strain K56-2, a representative of the Burkholderia cepacia complex, is part of the epidemic and clinically problematic ET12 lineage. The strain produced plant tissue watersoaking (ptw) on onion tissue, which is a plant disease-associated trait. Using plasposon mutagenesis, mutants in the ptw phenotype were generated. The translated sequence of a disrupted gene (ptwD4) from a ptw-negative mutant showed homology to VirD4-like proteins. Analysis of the region proximal to the transfer gene homolog identified a gene cluster located on the 92-kb resident plasmid that showed homology to type IV secretion systems. The role of ptwD4, ptwC, ptwB4, and ptwB10 in the expression of ptw activity was determined by conducting site-directed mutagenesis. The ptw phenotype was not expressed by K56-2 derivatives with a disruption in ptwD4, ptwB4, or ptwB10 but was observed in a derivative with a disruption in ptwC. Complementation of ptw-negative K56-2 derivatives in trans resulted in complete restoration of the ptw phenotype. In addition, analysis of culture supernatants revealed that the putative ptw effector(s) was a secreted, heat-stable protein(s) that caused plasmolysis of plant protoplasts. A second chromosomally encoded type IV secretion system with complete homology to the VirB-VirD system was identified in K56-2. Site-directed mutagenesis of key secretory genes in the VirB-VirD system did not affect expression of the ptw phenotype. Our findings indicate that in strain K56-2, the plasmid-encoded Ptw type IV secretion system is responsible for the secretion of a plant cytotoxic protein(s). | lld:pubmed |
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| pubmed-article:15342570 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15342570 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15342570 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15342570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15342570 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15342570 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:15342570 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:15342570 | pubmed:author | pubmed-author:Mahenthiralin... | lld:pubmed |
| pubmed-article:15342570 | pubmed:author | pubmed-author:LiPumaJohn... | lld:pubmed |
| pubmed-article:15342570 | pubmed:author | pubmed-author:GonzalezCarlo... | lld:pubmed |
| pubmed-article:15342570 | pubmed:author | pubmed-author:EngledowAmand... | lld:pubmed |
| pubmed-article:15342570 | pubmed:author | pubmed-author:MedranoEnriqu... | lld:pubmed |
| pubmed-article:15342570 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15342570 | pubmed:volume | 186 | lld:pubmed |
| pubmed-article:15342570 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15342570 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15342570 | pubmed:pagination | 6015-24 | lld:pubmed |
| pubmed-article:15342570 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:15342570 | pubmed:meshHeading | pubmed-meshheading:15342570... | lld:pubmed |
| pubmed-article:15342570 | pubmed:meshHeading | pubmed-meshheading:15342570... | lld:pubmed |
| pubmed-article:15342570 | pubmed:meshHeading | pubmed-meshheading:15342570... | lld:pubmed |
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| pubmed-article:15342570 | pubmed:meshHeading | pubmed-meshheading:15342570... | lld:pubmed |
