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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017262,
umls-concept:C0021469,
umls-concept:C0023418,
umls-concept:C0026473,
umls-concept:C0086418,
umls-concept:C0600531,
umls-concept:C0812380,
umls-concept:C0813971,
umls-concept:C0851285,
umls-concept:C0919524,
umls-concept:C1171362,
umls-concept:C1280500,
umls-concept:C1425353,
umls-concept:C1510411,
umls-concept:C1511938,
umls-concept:C1515670,
umls-concept:C1521761,
umls-concept:C1533157,
umls-concept:C1705691
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pubmed:issue |
17
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pubmed:dateCreated |
2004-9-2
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pubmed:abstractText |
TEL2 is a member of the ETS family of transcription factors, which is highly similar to TEL1/ETV6. It binds to DNA via the ETS domain and interacts with itself or TEL1 via the pointed domain. The expression of TEL2 in normal and leukemic hematopoietic cells suggests a role in hematopoietic development. In this article, we describe the role of TEL2 in hematopoietic differentiation and cellular transformation. Quantitative reverse transcription-PCR showed that the expression of TEL2 mRNA was down-regulated during monocytic differentiation of U937 and HL60 induced by 1,25-(OH)2 vitamin D3 and 12-O-tetradecanoylphorbol 13-acetate, respectively. Overexpression of TEL2 in U937 cells inhibited differentiation induced by vitamin D3. In contrast, overexpression of a TEL2 mutant lacking either the pointed domain or a functional ETS domain induced both differentiation of U937 cells and inhibited their growth in vitro and in vivo. In addition, these mutants blocked TEL2-mediated transcriptional repression of a synthetic promoter containing TEL2 binding sites. These data suggest that dominant-negative inhibition of TEL2 might cause differentiation. Quantitative reverse transcription-PCR demonstrated that TEL2 is expressed at higher level in some primary human leukemia samples than in normal bone marrow. Furthermore, overexpression of TEL2 in NIH3T3-UCLA cells blocked the inhibitory effect of TEL1 on Ras-induced cellular transformation. These results suggest that TEL2 may play an important role in hematopoiesis and oncogenesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ELF2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ETS translocation variant 6 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-ets,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ras Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6091-100
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15342392-Animals,
pubmed-meshheading:15342392-Calcitriol,
pubmed-meshheading:15342392-Cell Differentiation,
pubmed-meshheading:15342392-Cell Transformation, Neoplastic,
pubmed-meshheading:15342392-DNA-Binding Proteins,
pubmed-meshheading:15342392-Gene Expression Regulation, Leukemic,
pubmed-meshheading:15342392-Hematopoietic Stem Cells,
pubmed-meshheading:15342392-Humans,
pubmed-meshheading:15342392-Leukemia, Myeloid,
pubmed-meshheading:15342392-Mice,
pubmed-meshheading:15342392-Monocytes,
pubmed-meshheading:15342392-Mutation,
pubmed-meshheading:15342392-NIH 3T3 Cells,
pubmed-meshheading:15342392-Proto-Oncogene Proteins c-ets,
pubmed-meshheading:15342392-RNA, Messenger,
pubmed-meshheading:15342392-Repressor Proteins,
pubmed-meshheading:15342392-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15342392-Transcription Factors,
pubmed-meshheading:15342392-Transcriptional Activation,
pubmed-meshheading:15342392-Transfection,
pubmed-meshheading:15342392-U937 Cells,
pubmed-meshheading:15342392-Up-Regulation,
pubmed-meshheading:15342392-ras Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
TEL2, an ETS factor expressed in human leukemia, regulates monocytic differentiation of U937 Cells and blocks the inhibitory effect of TEL1 on ras-induced cellular transformation.
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pubmed:affiliation |
Department of Genetics, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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