|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2004-9-2
|
|
pubmed:abstractText |
We demonstrate a role in oxidative and metal stress resistance for the MAPK-activated protein kinases Rck1 and Rck2 in Saccharomyces cerevisiae. We show that Hog1 is robustly phosphorylated in a Pbs2-dependent way during oxidative stress, and that Rck2 also is phosphorylated under these circumstances. Hog1 concentrates in the nucleus in oxidative stress. Hog1 localization is partially dependent on Rck2, as rck2 cells have more nuclear Hog1 than wild-type cells. We find several proteins with a role in oxidative stress resistance using Rck1 or Rck2 as baits in a two-hybrid screen. We identify the transcription factor Yap2 as a putative target for Rck1, and the Zn2+ transporter Zrc1 as a target for Rck2. Yap2 is normally cytoplasmic, but rapidly migrates to the nucleus upon exposure to oxidative stress agents. In a fraction of untreated pbs2 cells, Yap2 is nuclear. Zrc1 co-immunoprecipitates with Rck2, and ZRC1 is genetically downstream of RCK2. These data connect activation of the Hog1 MAPK cascade with effectors having a role in oxidative stress resistance.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CAD1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Cation Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HOG1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Metals,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PBS2 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RCK1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/RCK2 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ZRC1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/tert-Butylhydroperoxide
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0950-382X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
53
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1743-56
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:15341652-Animals,
pubmed-meshheading:15341652-Cation Transport Proteins,
pubmed-meshheading:15341652-Cell Nucleus,
pubmed-meshheading:15341652-Metals,
pubmed-meshheading:15341652-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:15341652-Mitogen-Activated Protein Kinases,
pubmed-meshheading:15341652-Oxidative Stress,
pubmed-meshheading:15341652-Phosphorylation,
pubmed-meshheading:15341652-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15341652-Saccharomyces cerevisiae,
pubmed-meshheading:15341652-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:15341652-Signal Transduction,
pubmed-meshheading:15341652-Transcription Factors,
pubmed-meshheading:15341652-Two-Hybrid System Techniques,
pubmed-meshheading:15341652-tert-Butylhydroperoxide
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance.
|
|
pubmed:affiliation |
Department of Cell and Molecular Biology, Lundberg Laboratory, Göteborg University, PO Box 462, SE-405 30 Göteborg, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
