Linked Life Data

15337303

Source:http://linkedlifedata.com/resource/pubmed/id/15337303

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2004-8-31
pubmed:abstractText
The alpha-subunits of Kv1 channels display characteristic distributions and restricted co-assembly in mammalian brain. The heterogeneous composition of Kv1 channels has made it difficult to use specific toxins to label brain structures. We used autoradiography to analyse the competitive behaviour of three Kv1 channel toxins--alpha-dendrotoxin, kaliotoxin, and mast cell degranulating peptide--for binding to kaliotoxin binding sites in various brain structures. IC(50) varied considerably between brain regions (by up to three orders of magnitude) for each ligand. alpha-dendrotoxin and kaliotoxin competed equally in some regions and to different extents in others, identifying two types of structure. Mast cell degranulating peptide competed with (125)I-kaliotoxin less efficiently than alpha-dendrotoxin and kaliotoxin, in all regions. Thus, differences in the capacity of these three toxins to bind to kaliotoxin binding sites provide evidence of major differences in the composition of the Kv1 channels constituting the kaliotoxin binding sites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0197-0186
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1039-47
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Heterogeneous competition of Kv1 channel toxins with kaliotoxin for binding in rat brain: autoradiographic analysis.
pubmed:affiliation
ITIS, CNRS-UMR 6150, IFR Jean Roche, Faculté de Médecine de Marseille, Université de la Méditerranée, Boulevard Pierre Dramard 13916 Marseille, France.
pubmed:publicationType
Journal Article