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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-8-31
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pubmed:abstractText |
MexZ is a transcriptional regulator of the mexXY multidrug transporter operon, which confers aminoglycoside resistance on Pseudomonas aeruginosa. Highly purified MexZ showed direct binding with a specific site of the mexZ-mexX intergenic DNA when probed by a gel retardation assay. Both in vitro chemical cross-linking experiments and an in vivo two-hybrid expression system showed that the active form of MexZ, which is capable of binding the intergenic DNA, appeared to be a dimer. These results explain the mechanism by which MexZ represses transcription of the mexXY operon, but do not explain the substrate-induced hyperproduction of MexXY. The presence of inducer antibiotic in the gel-retardation assay mixture failed to detect altered MexZ-probe DNA interaction suggesting the possible involvement of an additional regulator.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoglycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Intergenic,
http://linkedlifedata.com/resource/pubmed/chemical/MexXY protein, Pseudomonas...,
http://linkedlifedata.com/resource/pubmed/chemical/MexZ protein, Pseudomonas aeruginosa,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
238
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15336398-Amino Acid Sequence,
pubmed-meshheading:15336398-Aminoglycosides,
pubmed-meshheading:15336398-Anti-Bacterial Agents,
pubmed-meshheading:15336398-Bacterial Proteins,
pubmed-meshheading:15336398-Base Sequence,
pubmed-meshheading:15336398-DNA, Bacterial,
pubmed-meshheading:15336398-DNA, Intergenic,
pubmed-meshheading:15336398-Dimerization,
pubmed-meshheading:15336398-Drug Resistance, Multiple, Bacterial,
pubmed-meshheading:15336398-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:15336398-Gene Expression Regulation, Bacterial,
pubmed-meshheading:15336398-Molecular Sequence Data,
pubmed-meshheading:15336398-Operon,
pubmed-meshheading:15336398-Protein Binding,
pubmed-meshheading:15336398-Pseudomonas aeruginosa,
pubmed-meshheading:15336398-Repressor Proteins,
pubmed-meshheading:15336398-Transcription, Genetic,
pubmed-meshheading:15336398-Two-Hybrid System Techniques
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pubmed:year |
2004
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pubmed:articleTitle |
MexZ-mediated regulation of mexXY multidrug efflux pump expression in Pseudomonas aeruginosa by binding on the mexZ-mexX intergenic DNA.
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pubmed:affiliation |
Department of Molecular Life Science, Tokai University School of Medicine, 145 Shimokasuya, Isehara-shi, Kanagawa-ken 259-1193, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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