| pubmed-article:1533635 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C0018207 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C0062503 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C0311400 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:1533635 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:1533635 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:1533635 | pubmed:dateCreated | 1992-6-9 | lld:pubmed |
| pubmed-article:1533635 | pubmed:abstractText | The glycosylphosphatidylinositol (GPI)-anchor of the plasma membrane-associated heparan sulfate (HS) proteoglycan was metabolically radiolabeled with [3H]myristic acid, [3H]palmitic acid, [3H]inositol, [3H]ethanolamine, or [32P]phosphate in rat ovarian granulosa cell culture. Cell cultures labeled with [3H]myristic acid or [3H]palmitic acid were extracted with 4 M guanidine HCl buffer containing 2% Triton X-100 and the proteoglycans were purified by ion exchange chromatography after extensive delipidation. Specific incorporation of 3H into GPI-anchor was demonstrated by removing the label with a phosphatidylinositol-specific phospholipase C (PI-PLC). Incorporation of 3H activity into glycosaminoglycans and core glycoproteins was also demonstrated. However, the specific activity of 3H in these structures was approximately 2 orders of magnitude lower than that in the GPI-anchor, suggesting that 3H label was the result of the metabolic utilization of catabolic products of the 3H-labeled fatty acids. PI-PLC treatment of cell cultures metabolically labeled with [3H]inositol, [3H]ethanolamine, or [32P]phosphate specifically released radiolabeled cell surface-associated HS proteoglycans indicating the presence of GPI-anchor in these proteoglycans. GPI-anchored HS proteoglycans accounted for 20-30% of the total cell surface-associated HS proteoglycans and virtually all of them were removed by PI-PLC. These results further substantiate the presence of GPI-anchored heparan sulfate proteoglycan in ovarian granulosa cells and its cell surface localization. | lld:pubmed |
| pubmed-article:1533635 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1533635 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1533635 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1533635 | pubmed:month | May | lld:pubmed |
| pubmed-article:1533635 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1533635 | pubmed:author | pubmed-author:YanagishitaMM | lld:pubmed |
| pubmed-article:1533635 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1533635 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1533635 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1533635 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1533635 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1533635 | pubmed:pagination | 9499-504 | lld:pubmed |
| pubmed-article:1533635 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:1533635 | pubmed:meshHeading | pubmed-meshheading:1533635-... | lld:pubmed |
| pubmed-article:1533635 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1533635 | pubmed:articleTitle | Metabolic labeling of glycosylphosphatidylinositol-anchor of heparan sulfate proteoglycans in rat ovarian granulosa cells. | lld:pubmed |
| pubmed-article:1533635 | pubmed:affiliation | Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892. | lld:pubmed |
| pubmed-article:1533635 | pubmed:publicationType | Journal Article | lld:pubmed |
