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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1992-6-9
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pubmed:abstractText |
The glycosylphosphatidylinositol (GPI)-anchor of the plasma membrane-associated heparan sulfate (HS) proteoglycan was metabolically radiolabeled with [3H]myristic acid, [3H]palmitic acid, [3H]inositol, [3H]ethanolamine, or [32P]phosphate in rat ovarian granulosa cell culture. Cell cultures labeled with [3H]myristic acid or [3H]palmitic acid were extracted with 4 M guanidine HCl buffer containing 2% Triton X-100 and the proteoglycans were purified by ion exchange chromatography after extensive delipidation. Specific incorporation of 3H into GPI-anchor was demonstrated by removing the label with a phosphatidylinositol-specific phospholipase C (PI-PLC). Incorporation of 3H activity into glycosaminoglycans and core glycoproteins was also demonstrated. However, the specific activity of 3H in these structures was approximately 2 orders of magnitude lower than that in the GPI-anchor, suggesting that 3H label was the result of the metabolic utilization of catabolic products of the 3H-labeled fatty acids. PI-PLC treatment of cell cultures metabolically labeled with [3H]inositol, [3H]ethanolamine, or [32P]phosphate specifically released radiolabeled cell surface-associated HS proteoglycans indicating the presence of GPI-anchor in these proteoglycans. GPI-anchored HS proteoglycans accounted for 20-30% of the total cell surface-associated HS proteoglycans and virtually all of them were removed by PI-PLC. These results further substantiate the presence of GPI-anchored heparan sulfate proteoglycan in ovarian granulosa cells and its cell surface localization.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ethanolamine,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Heparan Sulfate Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Heparitin Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol,
http://linkedlifedata.com/resource/pubmed/chemical/Myristic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Myristic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9499-504
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1533635-Animals,
pubmed-meshheading:1533635-Cells, Cultured,
pubmed-meshheading:1533635-Chromatography, Gel,
pubmed-meshheading:1533635-Chromatography, Ion Exchange,
pubmed-meshheading:1533635-Ethanolamine,
pubmed-meshheading:1533635-Ethanolamines,
pubmed-meshheading:1533635-Female,
pubmed-meshheading:1533635-Glycolipids,
pubmed-meshheading:1533635-Glycosylphosphatidylinositols,
pubmed-meshheading:1533635-Granulosa Cells,
pubmed-meshheading:1533635-Heparan Sulfate Proteoglycans,
pubmed-meshheading:1533635-Heparitin Sulfate,
pubmed-meshheading:1533635-Inositol,
pubmed-meshheading:1533635-Kinetics,
pubmed-meshheading:1533635-Myristic Acid,
pubmed-meshheading:1533635-Myristic Acids,
pubmed-meshheading:1533635-Palmitic Acid,
pubmed-meshheading:1533635-Palmitic Acids,
pubmed-meshheading:1533635-Phosphates,
pubmed-meshheading:1533635-Phosphatidylinositols,
pubmed-meshheading:1533635-Phosphorus Radioisotopes,
pubmed-meshheading:1533635-Proteoglycans,
pubmed-meshheading:1533635-Rats,
pubmed-meshheading:1533635-Rats, Inbred Strains,
pubmed-meshheading:1533635-Sulfur Radioisotopes,
pubmed-meshheading:1533635-Tritium
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pubmed:year |
1992
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pubmed:articleTitle |
Metabolic labeling of glycosylphosphatidylinositol-anchor of heparan sulfate proteoglycans in rat ovarian granulosa cells.
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pubmed:affiliation |
Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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