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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-8-30
pubmed:abstractText
In vitro selection or systematic evolution of ligands by exponential enrichment is a combinatorial procedure that allows the identification of oligonucleotides showing properties of interest-so-called aptamers-through iterative selection/amplification rounds. Libraries containing as many as 1014 different sequences can be screened against a wide range of molecules. Ribonucleic acid (RNA), deoxyribonucleic acid (DNA), or chemically modified aptamers generally display high affinity and exquisite specificity of interaction with the target. Aptamers show a promising potential for diagnostic and therapeutic purposes. We describe here methods successfully used in our laboratory for the selection of RNA or DNA aptamers against an RNA structure (the transactivation response element of HIV-1) and a protein (the human ribonuclease H1).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
391-410
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins.
pubmed:affiliation
INSERM U386, Modulation Artificielledes Gènes Eucaryotes, Université Victor Segalen Bordeaux 2, France.
pubmed:publicationType
Journal Article