Source:http://linkedlifedata.com/resource/pubmed/id/15333466
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2004-12-1
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| pubmed:abstractText |
Expression of matrilysin-2, matrix metalloproteinase (MMP)-26, has been implicated in the progression of several types of human cancer. Matrilysin-2 has been reported to be a physiological and pathological activator of pro-MMP-9. The aim of this study was to examine matrilysin-2 expression and determine whether it is correlated with progression of human esophageal squamous cell carcinoma (ESCC). Semi-quantitative reverse transcriptase-polymerase chain reaction, immunohistochemical analysis, zymography and an in vitro invasion assay were performed. Matrilysin-2 mRNA expression was undetectable or only faintly detected in non-tumor tissues, but its overexpression was detected in 24 of the 50 ESCC tissues. Matrilysin-2 overexpression was significantly correlated with depth of invasion, lymph node metastasis and an advance in pathological tumor node metastasis (pTNM) stage. Sections with immunostaining signals in >10% of carcinoma cells at the invasive front, which were observed in 46 of 100 cases, were judged to be positive for matrilysin-2 expression. Matrilysin-2 expression was significantly correlated with depth of invasion, lymph node and distant metastasis, advance in pTNM stage and recurrence. Expression of matrilysin-2 was significantly correlated with nuclear beta-catenin expression and MMP-9 expression. Patients with matrilysin-2-positive cancer had significantly shorter overall and disease-free survival periods than did those with matrilysin-2-negative cancer. Matrilysin-2 expression retained its significant predictive value for overall and disease-free survival in multivariate analysis. Moreover, patients with concomitant expression of matrilysin-2 and MMP-9 had the worst prognosis. Zymography revealed that matrilysin-2 expression was significantly correlated with expression of active MMP-9 in ESCC tissues. Matrilysin-2-transfected TE-1 ESCC cells showed active MMP-9 activity and were more invasive in vitro compared with mock-transfected TE-1 cells. The results of this study suggest that matrilysin-2, the expression of which is closely correlated with nuclear beta-catenin expression and active MMP-9 activity, plays a key role in the progression of ESCC.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MMP26 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinases, Secreted,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0143-3334
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| pubmed:author |
pubmed-author:AdachiYasushiY,
pubmed-author:FujitaMasahiroM,
pubmed-author:HinodaYujiY,
pubmed-author:HirataTamakiT,
pubmed-author:HosokawaMasaoM,
pubmed-author:ImaiKohzohK,
pubmed-author:ImsumranArisaA,
pubmed-author:MiyamotoNobukiN,
pubmed-author:NoshoKatsuhikoK,
pubmed-author:TaniguchiHiroakiH,
pubmed-author:VinitketkumnuenAkravitA,
pubmed-author:YamamotoHiroyukiH
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| pubmed:issnType |
Print
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| pubmed:volume |
25
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2353-60
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15333466-Adenocarcinoma,
pubmed-meshheading:15333466-Carcinoma, Squamous Cell,
pubmed-meshheading:15333466-Cell Nucleus,
pubmed-meshheading:15333466-Cytoskeletal Proteins,
pubmed-meshheading:15333466-Disease Progression,
pubmed-meshheading:15333466-Enzyme Activation,
pubmed-meshheading:15333466-Esophageal Neoplasms,
pubmed-meshheading:15333466-Female,
pubmed-meshheading:15333466-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:15333466-Humans,
pubmed-meshheading:15333466-Immunoenzyme Techniques,
pubmed-meshheading:15333466-Lymphatic Metastasis,
pubmed-meshheading:15333466-Male,
pubmed-meshheading:15333466-Matrix Metalloproteinase 9,
pubmed-meshheading:15333466-Matrix Metalloproteinases,
pubmed-meshheading:15333466-Matrix Metalloproteinases, Secreted,
pubmed-meshheading:15333466-Middle Aged,
pubmed-meshheading:15333466-Neoplasm Invasiveness,
pubmed-meshheading:15333466-Neoplasm Recurrence, Local,
pubmed-meshheading:15333466-Prognosis,
pubmed-meshheading:15333466-RNA, Messenger,
pubmed-meshheading:15333466-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15333466-Survival Rate,
pubmed-meshheading:15333466-Trans-Activators,
pubmed-meshheading:15333466-Transfection,
pubmed-meshheading:15333466-beta Catenin
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| pubmed:year |
2004
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| pubmed:articleTitle |
Association of matrilysin-2 (MMP-26) expression with tumor progression and activation of MMP-9 in esophageal squamous cell carcinoma.
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| pubmed:affiliation |
First Department of Internal Medicine, Sapporo Medical University, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan. h-yama@sapmed.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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