Source:http://linkedlifedata.com/resource/pubmed/id/15329494
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2004-8-26
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pubmed:abstractText |
Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1341-7568
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-44
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pubmed:dateRevised |
2005-11-21
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pubmed:meshHeading |
pubmed-meshheading:15329494-Amino Acid Sequence,
pubmed-meshheading:15329494-Arabidopsis Proteins,
pubmed-meshheading:15329494-Cell Cycle,
pubmed-meshheading:15329494-Centromere,
pubmed-meshheading:15329494-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:15329494-Cloning, Molecular,
pubmed-meshheading:15329494-Conserved Sequence,
pubmed-meshheading:15329494-In Situ Hybridization, Fluorescence,
pubmed-meshheading:15329494-Molecular Sequence Data,
pubmed-meshheading:15329494-Sequence Homology, Amino Acid
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pubmed:year |
2004
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pubmed:articleTitle |
Characterization of a CENP-C homolog in Arabidopsis thaliana.
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pubmed:affiliation |
Research Institute for Bioresources, Okayama University, Kurashiki, Japan. yogura@rib.okayama-u.ac.jp
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pubmed:publicationType |
Journal Article
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