|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2004-8-25
|
|
pubmed:abstractText |
In mammals, heterochromatin is characterized by DNA methylation at CpG dinucleotides and methylation at lysine 9 of histone H3. It is currently unclear whether there is a coordinated transmission of these two epigenetic modifications through DNA replication. Here we show that the methyl-CpG binding protein MBD1 forms a stable complex with histone H3-K9 methylase SETDB1. Moreover, during DNA replication, MBD1 recruits SETDB1 to the large subunit of chromatin assembly factor CAF-1 to form an S phase-specific CAF-1/MBD1/SETDB1 complex that facilitates methylation of H3-K9 during replication-coupled chromatin assembly. In the absence of MBD1, H3-K9 methylation is lost at multiple genomic loci and results in activation of p53BP2 gene, normally repressed by MBD1 in HeLa cells. Our data suggest a model in which H3-K9 methylation by SETDB1 is dependent on MBD1 and is heritably maintained through DNA replication to support the formation of stable heterochromatin at methylated DNA.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CNOT8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Lysine,
http://linkedlifedata.com/resource/pubmed/chemical/MBD1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/SETDB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TP53BP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1097-2765
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
27
|
|
pubmed:volume |
15
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
595-605
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:15327775-Apoptosis Regulatory Proteins,
pubmed-meshheading:15327775-Carrier Proteins,
pubmed-meshheading:15327775-Chromatin Assembly and Disassembly,
pubmed-meshheading:15327775-CpG Islands,
pubmed-meshheading:15327775-DNA Replication,
pubmed-meshheading:15327775-DNA-Binding Proteins,
pubmed-meshheading:15327775-Gene Silencing,
pubmed-meshheading:15327775-HeLa Cells,
pubmed-meshheading:15327775-Histones,
pubmed-meshheading:15327775-Humans,
pubmed-meshheading:15327775-Lysine,
pubmed-meshheading:15327775-Macromolecular Substances,
pubmed-meshheading:15327775-Methylation,
pubmed-meshheading:15327775-Models, Biological,
pubmed-meshheading:15327775-Promoter Regions, Genetic,
pubmed-meshheading:15327775-Protein Methyltransferases,
pubmed-meshheading:15327775-Proteins,
pubmed-meshheading:15327775-RNA, Small Interfering,
pubmed-meshheading:15327775-S Phase,
pubmed-meshheading:15327775-Transcription Factors,
pubmed-meshheading:15327775-Two-Hybrid System Techniques
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly.
|
|
pubmed:affiliation |
School of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, United Kingdom.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
